biostudies-literatureXia XInnovation team of general Universities in Guangdong ProvinceGuangdong Basic and Applied Basic Research FoundationScience and Technology Program of GuangzhouNational Natural Science Foundation of China211https://www.ebi.ac.uk/biostudies/studies/S-EPMC12659323biostudies-literatureUnknown27(1)<h4>Background</h4>HER2-positive breast cancer is a prevalent pathological subtype of breast cancer. Resistance to anti-HER2 targeted therapies remains a significant challenge in treatment. Understanding the role of HER2 in breast cancer progression is essential.<h4>Methods</h4>The proteomics analysis was used to explore the regulated proteins in patients with HER2-positive breast cancer. MTS, EdU staininig, flow cytometry and colony formation assays were used to cell proliferation and apoptosis. Protein expressions and interaction of CAND1 and HER2 were clarified by western blot, immunofluorescence and co-immunoprecipitation experiments. In vivo studies using nude mice demonstrated the role of CAND1 in HER2-positive breast cancer cell growth.<h4>Results</h4>An increase in CAND1 expressionBreast cancer research : BCRCAND1 mediates CUL7-dependent HER2 protein stability to drive breast cancer progression.PMC126593232022KCXTD02182373327, 8240111911, 824030282023A03J04272021A1515110868Xia XLiao YTang MZhang JHe XHuang HChen YfalseCAND1 mediates CUL7-dependent HER2 protein stability to drive breast cancer progression.<h4>Background</h4>HER2-positive breast cancer is a prevalent pathological subtype of breast cancer. Resistance to anti-HER2 targeted therapies remains a significant challenge in treatment. Understanding the role of HER2 in breast cancer progression is essential.<h4>Methods</h4>The proteomics analysis was used to explore the regulated proteins in patients with HER2-positive breast cancer. MTS, EdU staininig, flow cytometry and colony formation assays were used to cell proliferation and apoptosis. Protein expressions and interaction of CAND1 and HER2 were clarified by western blot, immunofluorescence and co-immunoprecipitation experiments. In vivo studies using nude mice demonstrated the role of CAND1 in HER2-positive breast cancer cell growth.<h4>Results</h4>An increase in CAND1 expression2025-01-01T00:00:00Z2025 Nov2026-06-06T07:09:06.822Z2026-06-06T03:06:55.529ZS-EPMC126593234131079410.1186/s13058-025-02158-8