biostudies-literatureGervasi ANational Plan for NRRP Complementary Investments (PNC, established with the decree-law 6 May 2021, n. 59, converted by law n. 101 of 2021) in the call for the funding of research initiatives for technologies and innovative trajectories in the health and care sectors (Directorial Decree n. 931 of 06-06-2022)-AdvaNced Technologies for Human-centrEd Medicine - ANTHEMNational Biodiversity Future Center (NBFC) by the European Union NextGenerationEU, PNRR; META-CONNECT- Spoke 6512https://www.ebi.ac.uk/biostudies/studies/S-EPMC12659480biostudies-literatureUnknown23(1)<h4>Background</h4>Connexin 43 (CX43) is a hemichannel (HC)- and gap junction (GJ)-forming protein that mediates the exchange of small molecules between the intracellular and extracellular environments, as well as intercellular communication. In addition to this canonical role, recent studies have shown that its functions range from transcriptional regulation to intracellular homeostasis. The ability of CX43 to translocate into mitochondria suggests its involvement in energy metabolism. However, the functions of mitochondrial CX43 (mt-CX43) in neural cells remain unexplored.<h4>Methods</h4>Our study investigated the expression and localisation of mt-CX43 through western blot and immunofluorescence analyses in four immortalised human glioma cell lines: T98-G, A-172, CCF-STTG1, and U-87 MG. Cell communication and signaling : CCSMitochondrial connexin 43 modulates metabolic stress adaptation in glioma cell lines.PMC12659480CN00000033PNC0000003Denaro SD'Aprile SZappala ATibullo DGervasi AParenti RAmorini AMVicario NJorgacevski JPotokar MZorec RfalseMitochondrial connexin 43 modulates metabolic stress adaptation in glioma cell lines.<h4>Background</h4>Connexin 43 (CX43) is a hemichannel (HC)- and gap junction (GJ)-forming protein that mediates the exchange of small molecules between the intracellular and extracellular environments, as well as intercellular communication. In addition to this canonical role, recent studies have shown that its functions range from transcriptional regulation to intracellular homeostasis. The ability of CX43 to translocate into mitochondria suggests its involvement in energy metabolism. However, the functions of mitochondrial CX43 (mt-CX43) in neural cells remain unexplored.<h4>Methods</h4>Our study investigated the expression and localisation of mt-CX43 through western blot and immunofluorescence analyses in four immortalised human glioma cell lines: T98-G, A-172, CCF-STTG1, and U-87 MG. 2025-01-01T00:00:00Z2025 Nov2026-07-15T06:35:47.262Z2026-06-30T03:22:59.101ZS-EPMC126594804131064610.1186/s12964-025-02523-2