{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Ramatchandirin B"],"funding":["NICHD NIH HHS","NIDDK NIH HHS","NIAID NIH HHS","NHLBI NIH HHS","NIH HHS"],"pubmed_abstract":["<h4>Background</h4>Severe anemia is a common comorbidity in preterm infants in the neonatal intensive care unit, which is caused by phlebotomy, low erythropoietin levels, low red blood cell (RBC) lifespan, and exacerbated by the underlying erythropoietic immaturity. Anemia causes tissue hypoxia, which may alter the hematopoiesis niche in the liver. This study utilized our preclinical mouse model of phlebotomy-induced anemia (PIA) to investigate the immune cell atlas in the liver.<h4>Methods</h4>C57BL/6 mice were subjected to timed phlebotomy between postnatal days 2-10 to induce severe anemia. Immune cells in anemic liver were characterized by Single-cell (sc) RNA-sequencing and a flow cytometry approach.<h4>Results</h4>The scRNA-seq analysis revealed that PIA is associated with an altered"],"journal":["Pediatric research"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12659965"],"repository":["biostudies-literature"],"pubmed_title":["Immune landscape in liver of neonatal mice with phlebotomy-induced anemia."],"pmcid":["PMC12659965"],"funding_grant_id":["R21 HD105880","R01 HL163043","S10 OD028483","R01 AI171980","R01 HD116950","R01 DK129552"],"pubmed_authors":["Lawal ZD","Ramatchandirin B","Alnahhas Y","Subrramanya A","Konnikova L","MohanKumar K","Wang W","Balamurugan MA","Desiraju S","Tseng G","Ferris M","George Raj J"],"additional_accession":[]},"is_claimable":false,"name":"Immune landscape in liver of neonatal mice with phlebotomy-induced anemia.","description":"<h4>Background</h4>Severe anemia is a common comorbidity in preterm infants in the neonatal intensive care unit, which is caused by phlebotomy, low erythropoietin levels, low red blood cell (RBC) lifespan, and exacerbated by the underlying erythropoietic immaturity. Anemia causes tissue hypoxia, which may alter the hematopoiesis niche in the liver. This study utilized our preclinical mouse model of phlebotomy-induced anemia (PIA) to investigate the immune cell atlas in the liver.<h4>Methods</h4>C57BL/6 mice were subjected to timed phlebotomy between postnatal days 2-10 to induce severe anemia. Immune cells in anemic liver were characterized by Single-cell (sc) RNA-sequencing and a flow cytometry approach.<h4>Results</h4>The scRNA-seq analysis revealed that PIA is associated with an altered","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-31T03:16:34.066Z","creation":"2026-05-31T03:10:18.067Z"},"accession":"S-EPMC12659965","cross_references":{"pubmed":["40962864"],"doi":["10.1038/s41390-025-04361-x"]}}