{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sun G"],"funding":["NIAID NIH HHS"],"pagination":["15506-11"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1266084"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["102(43)"],"pubmed_abstract":["A number of insect effector genes activated by the steroid hormone 20-hydroxyecdysone (20E) are dually controlled by the ecdysteroid receptor (EcR/USP) and products of ecdysteroid early responsive genes (E74, E75, and Broad). However, the molecular mechanism of this dual action is poorly understood. Here we examined transcriptional activation of the vitellogenin (Vg) gene in the yellow fever mosquito, Aedes aegypti, by EcR/USP and E74 in response to an elevation of 20E titers. There are two isoforms of the Aedes E74 gene, AaE74A and AaE74B, which have a common C-terminal Ets DNA-binding domain and isoform-specific N termini in the female mosquito. Inhibiting expression of AaE74B but not AaE74A by RNA interference led to substantial reduction in the Vg gene expression. AaE74B and the ecdyst"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Synergistic action of E74B and ecdysteroid receptor in activating a 20-hydroxyecdysone effector gene."],"pmcid":["PMC1266084"],"funding_grant_id":["AI 36959","R01 AI036959"],"pubmed_authors":["Zhu J","Chen L","Raikhel AS","Sun G"],"additional_accession":[]},"is_claimable":false,"name":"Synergistic action of E74B and ecdysteroid receptor in activating a 20-hydroxyecdysone effector gene.","description":"A number of insect effector genes activated by the steroid hormone 20-hydroxyecdysone (20E) are dually controlled by the ecdysteroid receptor (EcR/USP) and products of ecdysteroid early responsive genes (E74, E75, and Broad). However, the molecular mechanism of this dual action is poorly understood. Here we examined transcriptional activation of the vitellogenin (Vg) gene in the yellow fever mosquito, Aedes aegypti, by EcR/USP and E74 in response to an elevation of 20E titers. There are two isoforms of the Aedes E74 gene, AaE74A and AaE74B, which have a common C-terminal Ets DNA-binding domain and isoform-specific N termini in the female mosquito. Inhibiting expression of AaE74B but not AaE74A by RNA interference led to substantial reduction in the Vg gene expression. AaE74B and the ecdyst","dates":{"release":"2005-01-01T00:00:00Z","publication":"2005 Oct","modification":"2026-03-16T16:11:58.11Z","creation":"2019-03-27T00:12:55Z"},"accession":"S-EPMC1266084","cross_references":{"pubmed":["16230625"],"doi":["10.1073/pnas.0503501102"]}}