{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(11)"],"submitter":["Palancade B"],"pubmed_abstract":["Using a genetic screen, we have identified a previously uncharacterized Saccharomyces cerevisiae open reading frame (renamed PML39) that displays a specific interaction with nucleoporins of the Nup84 complex. Localization of a Pml39-green fluorescent protein (GFP) fusion and two-hybrid studies revealed that Pml39 is mainly docked to a subset of nuclear pore complexes opposite to the nucleolus through interactions with Mlp1 and Mlp2. The absence of Pml39 leads to a specific leakage of unspliced mRNAs that is not enhanced upon MLP1 deletion. In addition, overexpression of PML39-GFP induces a specific trapping of mRNAs transcribed from an intron-containing reporter and of the heterogenous nuclear ribonucleoprotein Nab2 within discrete nuclear domains. In a nup60delta mutant, Pml39 is mislocal"],"journal":["Molecular biology of the cell"],"pagination":["5258-68"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1266424"],"repository":["biostudies-literature"],"pubmed_title":["Pml39, a novel protein of the nuclear periphery required for nuclear retention of improper messenger ribonucleoparticles."],"pmcid":["PMC1266424"],"pubmed_authors":["Loeillet S","Doye V","Zuccolo M","Palancade B","Nicolas A"],"additional_accession":[]},"is_claimable":false,"name":"Pml39, a novel protein of the nuclear periphery required for nuclear retention of improper messenger ribonucleoparticles.","description":"Using a genetic screen, we have identified a previously uncharacterized Saccharomyces cerevisiae open reading frame (renamed PML39) that displays a specific interaction with nucleoporins of the Nup84 complex. Localization of a Pml39-green fluorescent protein (GFP) fusion and two-hybrid studies revealed that Pml39 is mainly docked to a subset of nuclear pore complexes opposite to the nucleolus through interactions with Mlp1 and Mlp2. The absence of Pml39 leads to a specific leakage of unspliced mRNAs that is not enhanced upon MLP1 deletion. In addition, overexpression of PML39-GFP induces a specific trapping of mRNAs transcribed from an intron-containing reporter and of the heterogenous nuclear ribonucleoprotein Nab2 within discrete nuclear domains. In a nup60delta mutant, Pml39 is mislocal","dates":{"release":"2005-01-01T00:00:00Z","publication":"2005 Nov","modification":"2025-04-18T16:10:38.096Z","creation":"2019-03-27T01:25:22Z"},"accession":"S-EPMC1266424","cross_references":{"pubmed":["16162818"],"doi":["10.1091/mbc.e05-06-0527"]}}