biostudies-literatureUnknown16(11)Palancade BUsing a genetic screen, we have identified a previously uncharacterized Saccharomyces cerevisiae open reading frame (renamed PML39) that displays a specific interaction with nucleoporins of the Nup84 complex. Localization of a Pml39-green fluorescent protein (GFP) fusion and two-hybrid studies revealed that Pml39 is mainly docked to a subset of nuclear pore complexes opposite to the nucleolus through interactions with Mlp1 and Mlp2. The absence of Pml39 leads to a specific leakage of unspliced mRNAs that is not enhanced upon MLP1 deletion. In addition, overexpression of PML39-GFP induces a specific trapping of mRNAs transcribed from an intron-containing reporter and of the heterogenous nuclear ribonucleoprotein Nab2 within discrete nuclear domains. In a nup60delta mutant, Pml39 is mislocalMolecular biology of the cell5258-68https://www.ebi.ac.uk/biostudies/studies/S-EPMC1266424biostudies-literaturePml39, a novel protein of the nuclear periphery required for nuclear retention of improper messenger ribonucleoparticles.PMC1266424Loeillet SDoye VZuccolo MPalancade BNicolas AfalsePml39, a novel protein of the nuclear periphery required for nuclear retention of improper messenger ribonucleoparticles.Using a genetic screen, we have identified a previously uncharacterized Saccharomyces cerevisiae open reading frame (renamed PML39) that displays a specific interaction with nucleoporins of the Nup84 complex. Localization of a Pml39-green fluorescent protein (GFP) fusion and two-hybrid studies revealed that Pml39 is mainly docked to a subset of nuclear pore complexes opposite to the nucleolus through interactions with Mlp1 and Mlp2. The absence of Pml39 leads to a specific leakage of unspliced mRNAs that is not enhanced upon MLP1 deletion. In addition, overexpression of PML39-GFP induces a specific trapping of mRNAs transcribed from an intron-containing reporter and of the heterogenous nuclear ribonucleoprotein Nab2 within discrete nuclear domains. In a nup60delta mutant, Pml39 is mislocal2005-01-01T00:00:00Z2005 Nov2025-04-18T16:10:38.096Z2019-03-27T01:25:22ZS-EPMC12664241616281810.1091/mbc.e05-06-0527