biostudies-literatureUnknown99(15)McKibbin RSThe maize (Zea mays) Viviparous 1 (Vp1) transcription factor has been shown previously to be a major regulator of seed development, simultaneously activating embryo maturation and repressing germination. Hexaploid bread wheat (Triticum aestivum) caryopses are characterized by relatively weak embryo dormancy and are susceptible to preharvest sprouting (PHS), a phenomenon that is phenotypically similar to the maize vp1 mutation. Analysis of Vp-1 transcript structure in wheat embryos during grain development showed that each homeologue produces cytoplasmic mRNAs of different sizes. The majority of transcripts are spliced incorrectly, contain insertions of intron sequences or deletions of coding region, and do not have the capacity to encode full-length proteins. Several VP-1-related lower molProceedings of the National Academy of Sciences of the United States of America10203-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC126648biostudies-literatureTranscripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species.PMC126648Gale MDHoldsworth MJMcKibbin RSLazzeri PABailey PCAndrew LMFlintham JEWilkinson MDLenton JRfalseTranscripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species.The maize (Zea mays) Viviparous 1 (Vp1) transcription factor has been shown previously to be a major regulator of seed development, simultaneously activating embryo maturation and repressing germination. Hexaploid bread wheat (Triticum aestivum) caryopses are characterized by relatively weak embryo dormancy and are susceptible to preharvest sprouting (PHS), a phenomenon that is phenotypically similar to the maize vp1 mutation. Analysis of Vp-1 transcript structure in wheat embryos during grain development showed that each homeologue produces cytoplasmic mRNAs of different sizes. The majority of transcripts are spliced incorrectly, contain insertions of intron sequences or deletions of coding region, and do not have the capacity to encode full-length proteins. Several VP-1-related lower mol2002-01-01T00:00:00Z2002 Jul2025-04-05T16:14:26.392Z2019-03-27T00:17:16ZS-EPMC1266481211940810.1073/pnas.152318599