{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xu Y"],"funding":["Chinese Academy of Sciences","Key Technologies Research and Development Program","Natural Science Foundation of Beijing Municipality","China Postdoctoral Science Foundation","National Natural Science Foundation of China"],"pagination":["e04485"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12667501"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(44)"],"pubmed_abstract":["Intestinal epithelium is derived from Lgr5<sup>+</sup> intestinal stem cells (ISCs) located at the crypt base. However, how small nucleolar RNAs (snoRNAs) regulate ISC stemness remains elusive. Here, a conserved snoRNA, Snord15b, that is highly expressed in ISCs is identified. Snord15b knockout abolishes the self-renewal capacity of ISCs and impairs epithelial regeneration. Mechanistically, Snord15b interacts with interleukin enhancer-binding factor 2 (Ilf2) to recruit splicing factors, which mediates alternative splicing of Btrc to form a short isoform of Btrc, resulting in abrogation of E3 ligase complex formation for ubiquitination of β-catenin. Subsequently, stable β-catenin translocates into the nucleus of ISCs for activation of the Wnt/β-catenin signaling pathway, leading to ISC stem"],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pubmed_title":["Snord15b Maintains Stemness of Intestinal Stem Cells via Enhancement of Alternative Splicing of Btrc Short Isoform for Suppression of β-Catenin Ubiquitination."],"pmcid":["PMC12667501"],"funding_grant_id":["82130088","5222023","XDB0570301","2024M753424","Z231100007223013","2022YFF0710700","2020YFA0803501","82271785","2020M680712","2022T150685"],"pubmed_authors":["Li C","Fan Z","Du Y","Lan Y","Zhang P","Xiong Z","Fan H","Guo H","Tian Y","Wu R","Zhu X","Wang Z","Xu Y","Fan D"],"additional_accession":[]},"is_claimable":false,"name":"Snord15b Maintains Stemness of Intestinal Stem Cells via Enhancement of Alternative Splicing of Btrc Short Isoform for Suppression of β-Catenin Ubiquitination.","description":"Intestinal epithelium is derived from Lgr5<sup>+</sup> intestinal stem cells (ISCs) located at the crypt base. However, how small nucleolar RNAs (snoRNAs) regulate ISC stemness remains elusive. Here, a conserved snoRNA, Snord15b, that is highly expressed in ISCs is identified. Snord15b knockout abolishes the self-renewal capacity of ISCs and impairs epithelial regeneration. Mechanistically, Snord15b interacts with interleukin enhancer-binding factor 2 (Ilf2) to recruit splicing factors, which mediates alternative splicing of Btrc to form a short isoform of Btrc, resulting in abrogation of E3 ligase complex formation for ubiquitination of β-catenin. Subsequently, stable β-catenin translocates into the nucleus of ISCs for activation of the Wnt/β-catenin signaling pathway, leading to ISC stem","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T21:03:58.643Z","creation":"2026-05-21T03:13:00.424Z"},"accession":"S-EPMC12667501","cross_references":{"pubmed":["40884233"],"doi":["10.1002/advs.202504485"]}}