{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Cui J"],"funding":["NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Cystobactamids are non-ribosomal peptide natural products that function as DNA gyrase inhibitors, exhibiting significant antibacterial activity. They are isolated from Cystobacter sp. Cbv34 and contain various alkoxy groups on para-aminobenzoic acid moieties, which are believed to play a crucial role in antibacterial functions. The alkoxy groups are generated by iterative methylations on a methoxy group by the cobalamin (Cbl)-dependent radical S-adenosylmethionine (SAM) enzyme CysS. CysS catalyzes up to three methylations to give ethoxy, isopropoxy, sec-butoxy, and tert-butoxy groups. For each methylation, CysS uses a ping-pong mechanism in which two molecules of SAM are consumed. One SAM is used to methylate cob(I)alamin, while another generates a 5'-deoxyadenosyl 5'-radical to initiate s"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.11.17.688841"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12667779"],"repository":["biostudies-literature"],"pubmed_title":["Structural Basis for Iterative Methylation by a Cobalamin-dependent Radical S-Adenosylmethionine Enzyme in Cystobactamids Biosynthesis."],"pmcid":["PMC12667779"],"funding_grant_id":["R35 GM122595","P30 GM124169","S10 OD012289"],"pubmed_authors":["Wang B","Booker SJ","Cui J","Maurya RK"],"additional_accession":[]},"is_claimable":false,"name":"Structural Basis for Iterative Methylation by a Cobalamin-dependent Radical S-Adenosylmethionine Enzyme in Cystobactamids Biosynthesis.","description":"Cystobactamids are non-ribosomal peptide natural products that function as DNA gyrase inhibitors, exhibiting significant antibacterial activity. They are isolated from Cystobacter sp. Cbv34 and contain various alkoxy groups on para-aminobenzoic acid moieties, which are believed to play a crucial role in antibacterial functions. The alkoxy groups are generated by iterative methylations on a methoxy group by the cobalamin (Cbl)-dependent radical S-adenosylmethionine (SAM) enzyme CysS. CysS catalyzes up to three methylations to give ethoxy, isopropoxy, sec-butoxy, and tert-butoxy groups. For each methylation, CysS uses a ping-pong mechanism in which two molecules of SAM are consumed. One SAM is used to methylate cob(I)alamin, while another generates a 5'-deoxyadenosyl 5'-radical to initiate s","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-16T03:17:55.698Z","creation":"2026-06-16T03:09:53.491Z"},"accession":"S-EPMC12667779","cross_references":{"pubmed":["41332741"],"doi":["10.1101/2025.11.17.688841"]}}