{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Oz N"],"funding":["NIA NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Aging results from the gradual accumulation of molecular damage as a result of cellular processes and is characterized by impaired functions, most notably an age-related decline in ATP production. However, the causal relationship between cellular ATP homeostasis and aging has not been established. In this study, we used a novel approach by harnessing a nucleotide transporter from a eukaryotic intracellular parasite to facilitate the direct import of extracellular ATP into budding yeast cells, enabling us to effectively manipulate their intracellular ATP levels. We found that depletion of ATP significantly reduces lifespan, while the supplementation of ATP in the growth medium fully restores it thereby extending lifespan. Moreover, gene expression analysis revealed that elevated ATP levels "],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.04.14.648769"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12667902"],"repository":["biostudies-literature"],"pubmed_title":["Engineering ATP Import in Yeast Uncovers a Synthetic Route to Extend Cellular Lifespan."],"pmcid":["PMC12667902"],"funding_grant_id":["R01 GM144595","R35 GM150858","R01 AG086348","R01 AG038004"],"pubmed_authors":["Hao N","Labunskyy VM","Sari V","Song JH","Kaya A","Gladyshev VN","Prosser DC","Oz N","Patnaik P","Su H","Hameed R"],"additional_accession":[]},"is_claimable":false,"name":"Engineering ATP Import in Yeast Uncovers a Synthetic Route to Extend Cellular Lifespan.","description":"Aging results from the gradual accumulation of molecular damage as a result of cellular processes and is characterized by impaired functions, most notably an age-related decline in ATP production. However, the causal relationship between cellular ATP homeostasis and aging has not been established. In this study, we used a novel approach by harnessing a nucleotide transporter from a eukaryotic intracellular parasite to facilitate the direct import of extracellular ATP into budding yeast cells, enabling us to effectively manipulate their intracellular ATP levels. We found that depletion of ATP significantly reduces lifespan, while the supplementation of ATP in the growth medium fully restores it thereby extending lifespan. Moreover, gene expression analysis revealed that elevated ATP levels ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-10T03:13:57.858Z","creation":"2026-06-10T03:07:47.342Z"},"accession":"S-EPMC12667902","cross_references":{"pubmed":["41332595"],"doi":["10.1101/2025.04.14.648769"]}}