biostudies-literatureSeemiller LRPennsylvania State UniversityNIDA NIH HHSNational Institute on Drug AbuseNational Institute of General Medical SciencesNIGMS NIH HHS2022-2034https://www.ebi.ac.uk/biostudies/studies/S-EPMC12667965biostudies-literatureUnknown48(11)<h4>Background</h4>Adolescent sensitivity to alcohol is influenced by genetic background. Data from our laboratory suggested that adolescent C57BL/6J and DBA/2J inbred mice differed in susceptibility to alcohol-induced deficits in dorsal hippocampus-dependent contextual fear learning.<h4>Methods</h4>To investigate the biological underpinnings of this strain difference, we examined dorsal hippocampus gene expression using RNA-sequencing after alcohol or saline administration followed by Pavlovian fear conditioning across male and female C57BL/6J and DBA/2J adolescents.<h4>Results</h4>Strains exhibited dramatic differences in dorsal hippocampus gene expression. Specifically, C57BL/6J and DBA/2J strains differed by 3526 transcripts in males and 2675 transcripts in females. We identified pathwAlcohol, clinical & experimental researchAlcohol and fear conditioning produce strain-specific changes in the dorsal hippocampal transcriptome of adolescent C57BL/6J and DBA/2J mice.PMC12667965T32 GM108563R01 DA044339U01DA041632U01 DA041632T32GM108563U01DA044339Seemiller LRPraul CSiegel SRAlbert IGoldberg LRBryant CDBeierle JSebastian AZeid DGould TJfalseAlcohol and fear conditioning produce strain-specific changes in the dorsal hippocampal transcriptome of adolescent C57BL/6J and DBA/2J mice.<h4>Background</h4>Adolescent sensitivity to alcohol is influenced by genetic background. Data from our laboratory suggested that adolescent C57BL/6J and DBA/2J inbred mice differed in susceptibility to alcohol-induced deficits in dorsal hippocampus-dependent contextual fear learning.<h4>Methods</h4>To investigate the biological underpinnings of this strain difference, we examined dorsal hippocampus gene expression using RNA-sequencing after alcohol or saline administration followed by Pavlovian fear conditioning across male and female C57BL/6J and DBA/2J adolescents.<h4>Results</h4>Strains exhibited dramatic differences in dorsal hippocampus gene expression. Specifically, C57BL/6J and DBA/2J strains differed by 3526 transcripts in males and 2675 transcripts in females. We identified pathw2024-01-01T00:00:00Z2024 Nov2026-07-02T03:17:06.663Z2026-07-02T03:09:02.516ZS-EPMC126679653927966310.1111/acer.15440