biostudies-literatureUnknown46De Francesco FAutologous fat grafting has become integral to breast reconstruction, yet variability in graft retention continues to challenge its reliability. This study aimed to characterize the biological and clinical properties of adipose tissue harvested using Body-Jet water-assisted liposuction (WAL) technology. A cohort of 171 patients undergoing 206 lipofilling procedures was analyzed, alongside in vitro studies evaluating adipose-derived cell viability, proliferative capacity, and molecular profile. Lipoaspirates obtained using Body-Jet demonstrated high cellular viability, robust mesenchymal stem cell proliferation (doubling time ∼15 hours), and consistent trilineage differentiation (adipogenic, osteogenic, and chondrogenic). Gene expression and secretome analyses revealed a regenerative molecuJPRAS open750-769https://www.ebi.ac.uk/biostudies/studies/S-EPMC12670530biostudies-literatureStemness and clinical performance of water-jet technology: A translational study in breast reconstruction.PMC12670530Moro GPin ADCordaro ERCavaleri MPParodi PCFerraro AGZingaretti NRiccio MDe Francesco FFerraro GAQuaglia DZavan BfalseStemness and clinical performance of water-jet technology: A translational study in breast reconstruction.Autologous fat grafting has become integral to breast reconstruction, yet variability in graft retention continues to challenge its reliability. This study aimed to characterize the biological and clinical properties of adipose tissue harvested using Body-Jet water-assisted liposuction (WAL) technology. A cohort of 171 patients undergoing 206 lipofilling procedures was analyzed, alongside in vitro studies evaluating adipose-derived cell viability, proliferative capacity, and molecular profile. Lipoaspirates obtained using Body-Jet demonstrated high cellular viability, robust mesenchymal stem cell proliferation (doubling time ∼15 hours), and consistent trilineage differentiation (adipogenic, osteogenic, and chondrogenic). Gene expression and secretome analyses revealed a regenerative molecu2025-01-01T00:00:00Z2025 Dec2026-06-06T03:16:25.182Z2026-06-06T03:06:43.431ZS-EPMC126705304134196610.1016/j.jpra.2025.10.010