biostudies-literatureUnknown15Huang J<h4>Background</h4>Patients with haematological malignancies are immunocompromised and prone to respiratory infections, but identification of causative pathogens is challenging. The aim of this study was to analyse the ability of targeted next-generation sequencing (tNGS) to detect pathogens in immunocompromised patients.<h4>Methods</h4>tNGS and conventional microbiological tests (CMT) were performed on samples from the respiratory tract of 99 patients with suspected respiratory infections. Metagenomic next-generation sequencing (mNGS) was conducted in parallel in 43 patients. Comparative analysis was conducted using the Pearson χ2 test and Fisher's exact test, as appropriate.<h4>Results</h4>The overall microbial detection rates for tNGS were 100% (23/23) in the upper respiratory tract andFrontiers in cellular and infection microbiology1678858https://www.ebi.ac.uk/biostudies/studies/S-EPMC12672896biostudies-literatureTargeted next-generation sequencing for respiratory infections in patients with haematological malignancies.PMC12672896Pan ZLi SZhu JHuang JZhang ZHu XChen WJiang CWang LfalseTargeted next-generation sequencing for respiratory infections in patients with haematological malignancies.<h4>Background</h4>Patients with haematological malignancies are immunocompromised and prone to respiratory infections, but identification of causative pathogens is challenging. The aim of this study was to analyse the ability of targeted next-generation sequencing (tNGS) to detect pathogens in immunocompromised patients.<h4>Methods</h4>tNGS and conventional microbiological tests (CMT) were performed on samples from the respiratory tract of 99 patients with suspected respiratory infections. Metagenomic next-generation sequencing (mNGS) was conducted in parallel in 43 patients. Comparative analysis was conducted using the Pearson χ2 test and Fisher's exact test, as appropriate.<h4>Results</h4>The overall microbial detection rates for tNGS were 100% (23/23) in the upper respiratory tract and2025-01-01T00:00:00Z20252026-06-05T23:08:43.367Z2026-05-23T03:12:43.462ZS-EPMC126728964134636010.3389/fcimb.2025.1678858