{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fahrlander J"],"funding":["Deutsche Forschungsgemeinschaft","Deutsche Forschungsgemeinschaft (German Research Foundation)"],"pagination":["1739"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12673129"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["Centrosome amplification is a hallmark of colorectal cancer (CRC), yet its molecular origins remain elusive. Protein Kinase A (PKA), anchored to centrosomes, regulates a number of mitotic processes, though its role in maintaining centrosome integrity remains poorly understood. Here, we show that PKA is central to a signaling cascade involving the G protein-coupled estrogen receptor GPER1, which is essential for centrosome integrity in colon cancer cells. Activation of GPER1 by estrogens or a specific agonist triggers Gαs protein-mediated stimulation of adenylyl cyclase, elevating cAMP levels and thereby increasing PKA activity. Excessive activation of GPER1-PKA leads to extra centrosomes with enlarged, displaced Centrin-2 foci. Recruited to centrosomes by AKAP450, PKA phosphorylates Centri"],"journal":["Communications biology"],"pubmed_title":["A GPER-PKA-Centrin axis regulates centrosome numbers and centriole integrity in colon cancer cells."],"pmcid":["PMC12673129"],"funding_grant_id":["465732234; STO 1306/2-1"],"pubmed_authors":["Buhler M","Busse M","Becker M","Shih JM","Stolz A","Fahrlander J","Schonfelder G","Zordick C"],"additional_accession":[]},"is_claimable":false,"name":"A GPER-PKA-Centrin axis regulates centrosome numbers and centriole integrity in colon cancer cells.","description":"Centrosome amplification is a hallmark of colorectal cancer (CRC), yet its molecular origins remain elusive. Protein Kinase A (PKA), anchored to centrosomes, regulates a number of mitotic processes, though its role in maintaining centrosome integrity remains poorly understood. Here, we show that PKA is central to a signaling cascade involving the G protein-coupled estrogen receptor GPER1, which is essential for centrosome integrity in colon cancer cells. Activation of GPER1 by estrogens or a specific agonist triggers Gαs protein-mediated stimulation of adenylyl cyclase, elevating cAMP levels and thereby increasing PKA activity. Excessive activation of GPER1-PKA leads to extra centrosomes with enlarged, displaced Centrin-2 foci. Recruited to centrosomes by AKAP450, PKA phosphorylates Centri","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-06T07:37:58.426Z","creation":"2026-06-06T03:06:38.401Z"},"accession":"S-EPMC12673129","cross_references":{"pubmed":["41299155"],"doi":["10.1038/s42003-025-09249-4"]}}