{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Niederkofler S"],"funding":["Knut och Alice Wallenbergs Stiftelse","Funda??o para a Ci?ncia e a Tecnologia","Vetenskapsr?det","Stiftelsen f?r Strategisk Forskning","European Regional Development Fund"],"pagination":["64219-64231"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12673520"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(47)"],"pubmed_abstract":["Lipid nanoparticles (LNPs) are widely used for RNA delivery, but their efficiency remains limited, largely due to poor endosomal escape. Upon administration, proteins bind to the surface of the LNPs, influencing cellular uptake and potentially altering their interfacial properties. Such alterations may also affect their interaction with endosomal membranes, thus influencing the critical endosomal escape step. Using fluorescence microscopy imaging with single-LNP resolution, this study investigates how incubation in 10% fetal bovine serum alters the PEG modification and mRNA content of LNPs, as well as how serum incubation-induced alterations influence the interaction between LNPs and an anionic supported lipid bilayer (SLB), serving as a simplistic mimic for the anionic lipid membrane of l"],"journal":["ACS applied materials & interfaces"],"pubmed_title":["Effects of Serum Incubation on Lipid Nanoparticle PEG Shedding, mRNA Retention, and Membrane Interactions."],"pmcid":["PMC12673520"],"funding_grant_id":["20190577","2022 05016","IRC150065","032520","SFRH/149/BD/14199/2019"],"pubmed_authors":["Niederkofler S","Emilsson G","Hook F","Lindfors L","Galenkamp NS","Aliakbarinodehi N","Jing Y","Silva BFB","Ferreira CJO","Sasanian N","Ulkoski D","Agnarsson B","Parkkila P","Lundberg D"],"additional_accession":[]},"is_claimable":false,"name":"Effects of Serum Incubation on Lipid Nanoparticle PEG Shedding, mRNA Retention, and Membrane Interactions.","description":"Lipid nanoparticles (LNPs) are widely used for RNA delivery, but their efficiency remains limited, largely due to poor endosomal escape. Upon administration, proteins bind to the surface of the LNPs, influencing cellular uptake and potentially altering their interfacial properties. Such alterations may also affect their interaction with endosomal membranes, thus influencing the critical endosomal escape step. Using fluorescence microscopy imaging with single-LNP resolution, this study investigates how incubation in 10% fetal bovine serum alters the PEG modification and mRNA content of LNPs, as well as how serum incubation-induced alterations influence the interaction between LNPs and an anionic supported lipid bilayer (SLB), serving as a simplistic mimic for the anionic lipid membrane of l","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T22:08:36.128Z","creation":"2026-05-22T03:14:59.111Z"},"accession":"S-EPMC12673520","cross_references":{"pubmed":["41234148"],"doi":["10.1021/acsami.5c17052"]}}