biostudies-literatureWang QJiangsu Disabled Persons’ FederationResearch funding for Specially Appointed Professor at Nanjing University of Traditional Chinese MedicineJiangsu Disabled Persons' Federation149https://www.ebi.ac.uk/biostudies/studies/S-EPMC12673773biostudies-literatureUnknown22(1)<h4>Background</h4>Neural stem cells (NSCs), crucial for brain function and repair, are disrupted by high-fructose diet (HFrD) in proliferation and survival, linking to neurogenesis impairment and neuropsychiatric risks. Mechanistic insights remain undefined.<h4>Methods</h4>Comprehensive behavioral assessments were conducted on HFrD mice, including the tail suspension test (TST) and sucrose preference test (SPT) for depressive-like behaviors, elevated plus maze (EPM) and open field test (OFT) for anxiety-like behaviors, as well as novel object recognition (NOR) and Morris water maze (MWM) for cognition. Hippocampal NSCs and newborn neurons were quantified by immunofluorescence, and fructose-treated NE-4C cells underwent RNA sequencing (RNA-seq) analysis coupled with measurements of prolifeNutrition & metabolismHigh-fructose diet induces depressive-like behaviors and short-term memory deficits through hippocampal neurogenesis impairment via neural stem cell dysfunction.PMC126737730130380190322025SC02020Wang QHu YFeng ZLi FTang CZhou FWang YXie PMu RfalseHigh-fructose diet induces depressive-like behaviors and short-term memory deficits through hippocampal neurogenesis impairment via neural stem cell dysfunction.<h4>Background</h4>Neural stem cells (NSCs), crucial for brain function and repair, are disrupted by high-fructose diet (HFrD) in proliferation and survival, linking to neurogenesis impairment and neuropsychiatric risks. Mechanistic insights remain undefined.<h4>Methods</h4>Comprehensive behavioral assessments were conducted on HFrD mice, including the tail suspension test (TST) and sucrose preference test (SPT) for depressive-like behaviors, elevated plus maze (EPM) and open field test (OFT) for anxiety-like behaviors, as well as novel object recognition (NOR) and Morris water maze (MWM) for cognition. Hippocampal NSCs and newborn neurons were quantified by immunofluorescence, and fructose-treated NE-4C cells underwent RNA sequencing (RNA-seq) analysis coupled with measurements of prolife2025-01-01T00:00:00Z2025 Dec2026-06-07T04:41:52.46Z2026-06-07T03:06:52.937ZS-EPMC126737734133176110.1186/s12986-025-01043-7