biostudies-literatureUnknown16Gong XL<h4>Introduction</h4>Duckweed represents a promising alternative protein source, yet enhancing its protein content remains essential for large-scale applications. This study investigated how high nitrogen and phosphorus stress from biogas slurry affects protein accumulation in Landoltia punctata and explored the underlying molecular regulatory mechanisms.<h4>Methods</h4><i>L. punctata</i> were cultivated in 1/5 strength Hoagland medium supplemented with 0-5% pig farm biogas slurry. The experimental groups showing the highest (4%) and lowest (0%) protein content were selected for integrated transcriptomic and proteomic analyses. Differentially expressed genes (DEGs) and proteins (DEPs) were identified and functionally characterized.<h4>Results</h4>Biogas slurry treatments significantly incrFrontiers in plant science1694864https://www.ebi.ac.uk/biostudies/studies/S-EPMC12676286biostudies-literaturePost-transcriptional regulation dominates protein biosynthesis in &lt;i&gt;Landoltia punctata&lt;/i&gt; under biogas slurry stress.PMC12676286Gong XLRan CLu TTZhou LLZhang SLZhou TSu HHLi JZLi JYfalsePost-transcriptional regulation dominates protein biosynthesis in &lt;i&gt;Landoltia punctata&lt;/i&gt; under biogas slurry stress.<h4>Introduction</h4>Duckweed represents a promising alternative protein source, yet enhancing its protein content remains essential for large-scale applications. This study investigated how high nitrogen and phosphorus stress from biogas slurry affects protein accumulation in Landoltia punctata and explored the underlying molecular regulatory mechanisms.<h4>Methods</h4><i>L. punctata</i> were cultivated in 1/5 strength Hoagland medium supplemented with 0-5% pig farm biogas slurry. The experimental groups showing the highest (4%) and lowest (0%) protein content were selected for integrated transcriptomic and proteomic analyses. Differentially expressed genes (DEGs) and proteins (DEPs) were identified and functionally characterized.<h4>Results</h4>Biogas slurry treatments significantly incr2025-01-01T00:00:00Z20252026-06-05T23:33:26.419Z2026-05-23T03:13:46.553ZS-EPMC126762864135835810.3389/fpls.2025.1694864