biostudies-literatureErcumen AEunice Kennedy Shriver National Institute of Child Health and Human DevelopmentNICHD NIH HHS25173-25185https://www.ebi.ac.uk/biostudies/studies/S-EPMC12676737biostudies-literatureUnknown59(47)Soil can harbor enteropathogens and antimicrobial-resistant organisms from domestic animals. We enrolled 49 households with young children (28 soil floors and 21 concrete floors) in Bangladesh and recorded animal ownership/management. Staff swabbed the floor of children's sleeping area and collected floor dust and child hand rinses. We used IDEXX QuantiTray/2000 with and without cefotaxime supplementation to enumerate cefotaxime-resistant and generic E. coli. Soil floors had 40 times more dust than concrete (8.0 vs 0.2 g/m2, p-value = 0.005). We detected E. coli on 100% of soil vs 86% of concrete floors and cefotaxime-resistant E. coli on 89% of soil vs 43% of concrete floors (p-values <0.05). Cefotaxime-resistant E. coli prevalence on fEnvironmental science & technologyDirt Floors and Domestic Animals Are Associated with Soilborne Exposure to Antimicrobial-Resistant E. coli in Rural Bangladeshi Households.PMC12676737R01 HD108196R01HD108196Hossain MSRahman AHanif STabassum TMahmud ZHYeamin AHeitmann GBRahman MErcumen AMiah MRBenjamin-Chung JRahman MHAnderson CShoab AKHaque AJahan FTazin SfalseDirt Floors and Domestic Animals Are Associated with Soilborne Exposure to Antimicrobial-Resistant E. coli in Rural Bangladeshi Households.Soil can harbor enteropathogens and antimicrobial-resistant organisms from domestic animals. We enrolled 49 households with young children (28 soil floors and 21 concrete floors) in Bangladesh and recorded animal ownership/management. Staff swabbed the floor of children's sleeping area and collected floor dust and child hand rinses. We used IDEXX QuantiTray/2000 with and without cefotaxime supplementation to enumerate cefotaxime-resistant and generic E. coli. Soil floors had 40 times more dust than concrete (8.0 vs 0.2 g/m2, p-value = 0.005). We detected E. coli on 100% of soil vs 86% of concrete floors and cefotaxime-resistant E. coli on 89% of soil vs 43% of concrete floors (p-values <0.05). Cefotaxime-resistant E. coli prevalence on f2025-01-01T00:00:00Z2025 Dec2026-06-08T04:57:12.591Z2026-06-08T03:09:00.347ZS-EPMC126767374122182310.1021/acs.est.5c10329