biostudies-literatureUnknown361Wen RHuazhong Agricultural UniversityEscherichia coli phage vB_EcoS_P78, a novel bacteriophage isolated from swine feces, exhibits typical siphovirus morphology with an isometric head and a non-contractile tail, forming clear plaques approximately 4 mm in diameter. It demonstrated a broad host range, lysing 75 % of tested ESBL-producing and MDR E. coli clinical isolates across multiple serogroups. The phage displayed strong stability, retaining activity at temperatures up to 50 °C and within a pH range of 3-10. Results from one-step growth assays showed a 20-minute latent period and that each infected cell produced a burst size of 220 PFU. Whole-genome sequencing revealed a 44,574 bp dsDNA genome containing 58 ORFs, with no antibiotic resistance, and virulence genes detected. Phylogenetic analysis based on intergenomic similaVirus research199652https://www.ebi.ac.uk/biostudies/studies/S-EPMC12677095biostudies-literatureIsolation and characterization of a novel lytic bacteriophage vB_EcoS_P78 against multidrug-resistant Escherichia coli.PMC12677095Zheng JSun MPeng DTong JZheng CWen RLiu SfalseIsolation and characterization of a novel lytic bacteriophage vB_EcoS_P78 against multidrug-resistant Escherichia coli.Escherichia coli phage vB_EcoS_P78, a novel bacteriophage isolated from swine feces, exhibits typical siphovirus morphology with an isometric head and a non-contractile tail, forming clear plaques approximately 4 mm in diameter. It demonstrated a broad host range, lysing 75 % of tested ESBL-producing and MDR E. coli clinical isolates across multiple serogroups. The phage displayed strong stability, retaining activity at temperatures up to 50 °C and within a pH range of 3-10. Results from one-step growth assays showed a 20-minute latent period and that each infected cell produced a burst size of 220 PFU. Whole-genome sequencing revealed a 44,574 bp dsDNA genome containing 58 ORFs, with no antibiotic resistance, and virulence genes detected. Phylogenetic analysis based on intergenomic simila2025-01-01T00:00:00Z2025 Nov2026-07-05T03:12:22.311Z2026-07-05T03:08:25.581ZS-EPMC126770954117332710.1016/j.virusres.2025.199652