biostudies-literatureUnknown20(12)Paluri SCellular permeability barriers restrict the diffusion of solutes, pathogens, and cells across tissues. In Drosophila melanogaster, septate and tricellular junctions create permeability barriers in epithelia and the blood-brain barrier in glia. In vitro, and in vivo studies in the epithelia of the Drosophila wing imaginal discs identified microRNA-184 (miR-184) as a potential regulator of a subset of pleated septate and tricellular junction proteins. However, which tissues express miR-184, and the consequences of miR-184 expression on the blood-brain barrier has not been examined. Using a miR-184 sensor, we found that miR-184 is absent in tissues with pleated septate junctions but is present in tissues with smooth septate junctions. When expressed in the subperineurial glia that form the blPloS onee0328862https://www.ebi.ac.uk/biostudies/studies/S-EPMC12677480biostudies-literaturemicroRNA-184 distribution and consequences on glial septate junctions and the blood-brain barrier.PMC12677480Auld VJPaluri SfalsemicroRNA-184 distribution and consequences on glial septate junctions and the blood-brain barrier.Cellular permeability barriers restrict the diffusion of solutes, pathogens, and cells across tissues. In Drosophila melanogaster, septate and tricellular junctions create permeability barriers in epithelia and the blood-brain barrier in glia. In vitro, and in vivo studies in the epithelia of the Drosophila wing imaginal discs identified microRNA-184 (miR-184) as a potential regulator of a subset of pleated septate and tricellular junction proteins. However, which tissues express miR-184, and the consequences of miR-184 expression on the blood-brain barrier has not been examined. Using a miR-184 sensor, we found that miR-184 is absent in tissues with pleated septate junctions but is present in tissues with smooth septate junctions. When expressed in the subperineurial glia that form the bl2025-01-01T00:00:00Z20252026-07-15T13:51:48.373Z2026-07-05T03:11:29.666ZS-EPMC126774804134348410.1371/journal.pone.0328862