{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["van der Heijden FLAM"],"funding":["Dutch Research Council (NWO)"],"pubmed_abstract":["Adenosine diphosphate ribosylation is a significant post-translational modification implicated in various cellular processes and diseases, yet identifying its mono-ADP-ribose readers has posed considerable challenges. Previous proteomic screenings have predominantly focused on poly-ADP-ribose, resulting in the oversight of mono-ADP-ribose readers due to undefined ADP-ribose structures with randomly placed photo-crosslinking moieties. This study introduces novel, well-defined mono-ADP-ribose photoaffinity-based probes featuring distinct diazirine and benzophenone photo-crosslinkers aimed at selectively identifying mono-ADP-ribose readers. Using human HeLa protein extracts, these probes were employed in an interactomics screening, successfully uncovering numerous known and putative mono-ADP-"],"journal":["RSC chemical biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12679546"],"repository":["biostudies-literature"],"pubmed_title":["Identification of mono-ADP-ribose readers using well-defined photoaffinity-based probes."],"pmcid":["PMC12679546"],"funding_grant_id":["OCENW.KLEIN.497"],"pubmed_authors":["van der Heijden FLAM","Kondyli S","Filippov DV","Bleijerveld O","Vermeulen M","Weijers SA"],"additional_accession":[]},"is_claimable":false,"name":"Identification of mono-ADP-ribose readers using well-defined photoaffinity-based probes.","description":"Adenosine diphosphate ribosylation is a significant post-translational modification implicated in various cellular processes and diseases, yet identifying its mono-ADP-ribose readers has posed considerable challenges. Previous proteomic screenings have predominantly focused on poly-ADP-ribose, resulting in the oversight of mono-ADP-ribose readers due to undefined ADP-ribose structures with randomly placed photo-crosslinking moieties. This study introduces novel, well-defined mono-ADP-ribose photoaffinity-based probes featuring distinct diazirine and benzophenone photo-crosslinkers aimed at selectively identifying mono-ADP-ribose readers. Using human HeLa protein extracts, these probes were employed in an interactomics screening, successfully uncovering numerous known and putative mono-ADP-","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T23:35:11.783Z","creation":"2026-05-23T03:13:54.142Z"},"accession":"S-EPMC12679546","cross_references":{"pubmed":["41358196"],"doi":["10.1039/d5cb00176e"]}}