biostudies-literatureShen WBill and Melinda Gates Foundatione70628https://www.ebi.ac.uk/biostudies/studies/S-EPMC12680929biostudies-literatureUnknown124(5)Geminiviruses are single-stranded DNA viruses that infect many plant species and cause serious losses in agronomically important crops. An earlier study showed that the Arabidopsis thaliana ecotype Pla-1 is resistant to infection by diverse geminivirus species and mapped the major resistance locus Geminivirus Resistance of Pla-1 1 (GRP1) to chromosome 1. In this study, we fine-mapped the GRP1 locus to a 0.6-Mb region and showed that its strength is gene-dosage-dependent. We also uncovered two minor resistance loci, GRP2 and GRP3, that mapped to chromosomes 3 and 5, respectively, and showed that GRP3 resistance is dependent on GRP1. RNA-Seq analysis of plants inoculated with the geminivirus, cabbage leaf curl virus (CaLCuV, Begomovirus brassicae), showed that AT1G31540, which is located in The Plant journal : for cell and molecular biologyA TIR-NLR gene from Arabidopsis Pla-1 confers resistance to geminivirus infection.PMC12680929OPP1149990Wheeler ERajrani RNdunguru JDeppong DHanley-Bowdoin LMickelson-Young LAscencio-Ibanez JTReyes MIShen WfalseA TIR-NLR gene from Arabidopsis Pla-1 confers resistance to geminivirus infection.Geminiviruses are single-stranded DNA viruses that infect many plant species and cause serious losses in agronomically important crops. An earlier study showed that the Arabidopsis thaliana ecotype Pla-1 is resistant to infection by diverse geminivirus species and mapped the major resistance locus Geminivirus Resistance of Pla-1 1 (GRP1) to chromosome 1. In this study, we fine-mapped the GRP1 locus to a 0.6-Mb region and showed that its strength is gene-dosage-dependent. We also uncovered two minor resistance loci, GRP2 and GRP3, that mapped to chromosomes 3 and 5, respectively, and showed that GRP3 resistance is dependent on GRP1. RNA-Seq analysis of plants inoculated with the geminivirus, cabbage leaf curl virus (CaLCuV, Begomovirus brassicae), showed that AT1G31540, which is located in 2025-01-01T00:00:00Z2025 Dec2026-06-05T23:24:39.499Z2026-05-23T03:13:07.881ZS-EPMC126809294135132310.1111/tpj.70628