{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ciancone A"],"funding":["Frederick National Laboratory for Cancer Research","Foundation for the National Institutes of Health","National Cancer Institute","National Institutes of Health","Center for Cancer Research"],"pagination":["101315"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12682122"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(12)"],"pubmed_abstract":["Crosslinking mass spectrometry (MS) is a powerful approach for probing protein structures. However, most widely used crosslinkers rely on N-hydroxysuccinimide (NHS) esters, restricting reactivity primarily to lysine residues and protein N-termini, and rendering them incompatible with many amine-containing buffers (e.g., Tris) and key biochemical cofactors (e.g., ATP). To address these limitations, we introduce two novel vinyl-sulfone-based crosslinkers. Alkyne-BVSC is an enrichable, homobifunctional crosslinker featuring an acid-cleavable alkyne handle for downstream peptide enrichment. VSD is a heterobifunctional crosslinker combining a vinyl sulfone with a diazirine moiety for UV-activated photo-crosslinking. Both reagents are synthetically accessible from inexpensive precursors and reta"],"journal":["Molecular & cellular proteomics : MCP"],"pubmed_title":["Expanding the Crosslinking Mass Spectrometry Toolbox With Vinyl Sulfone Crosslinkers."],"pmcid":["PMC12682122"],"funding_grant_id":["HHSN261200800001E","Z01 BC011585 09"],"pubmed_authors":["Swenson RE","O'Reilly FJ","Schneekloth JS","Lee K","Sibley C","Ciancone A","Atallah-Yunes K","Spivey J","Wu H"],"additional_accession":[]},"is_claimable":false,"name":"Expanding the Crosslinking Mass Spectrometry Toolbox With Vinyl Sulfone Crosslinkers.","description":"Crosslinking mass spectrometry (MS) is a powerful approach for probing protein structures. However, most widely used crosslinkers rely on N-hydroxysuccinimide (NHS) esters, restricting reactivity primarily to lysine residues and protein N-termini, and rendering them incompatible with many amine-containing buffers (e.g., Tris) and key biochemical cofactors (e.g., ATP). To address these limitations, we introduce two novel vinyl-sulfone-based crosslinkers. Alkyne-BVSC is an enrichable, homobifunctional crosslinker featuring an acid-cleavable alkyne handle for downstream peptide enrichment. VSD is a heterobifunctional crosslinker combining a vinyl sulfone with a diazirine moiety for UV-activated photo-crosslinking. Both reagents are synthetically accessible from inexpensive precursors and reta","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-05T23:38:09.398Z","creation":"2026-05-23T03:13:54.978Z"},"accession":"S-EPMC12682122","cross_references":{"pubmed":["41130296"],"doi":["10.1016/j.mcpro.2025.101315"]}}