biostudies-literatureUnknown14(11)Gu Y<h4>Background</h4>Recent evidence highlights the importance of lipid metabolic reprogramming in lung adenocarcinoma (LUAD) progression. Due to the limitations of conventional techniques, the fine structure of lipids cannot be identified. The metabolic changes of lipid structural features-particularly carbon-carbon double bond (C=C) positional isomers-remain underexplored. This study aims to characterize the structural alterations of lipids, especially C=C positional isomers, in LUAD tissues to elucidate their potential roles in tumor progression.<h4>Methods</h4>We performed deep structural lipidomic profiling on paired normal lung (N) and LUAD (T) tissue samples using a combination of photochemical reaction-based structural analysis (Ω Analyzer) and liquid chromatography-mass spectrometryTranslational lung cancer research4768-4783https://www.ebi.ac.uk/biostudies/studies/S-EPMC12683375biostudies-literatureDeep structural lipidomic profiling reveals C=C positional isomers as potential biomarkers in lung adenocarcinoma tissue.PMC12683375Li XCui SPGu YShao MMHu BfalseDeep structural lipidomic profiling reveals C=C positional isomers as potential biomarkers in lung adenocarcinoma tissue.<h4>Background</h4>Recent evidence highlights the importance of lipid metabolic reprogramming in lung adenocarcinoma (LUAD) progression. Due to the limitations of conventional techniques, the fine structure of lipids cannot be identified. The metabolic changes of lipid structural features-particularly carbon-carbon double bond (C=C) positional isomers-remain underexplored. This study aims to characterize the structural alterations of lipids, especially C=C positional isomers, in LUAD tissues to elucidate their potential roles in tumor progression.<h4>Methods</h4>We performed deep structural lipidomic profiling on paired normal lung (N) and LUAD (T) tissue samples using a combination of photochemical reaction-based structural analysis (Ω Analyzer) and liquid chromatography-mass spectrometry2025-01-01T00:00:00Z2025 Nov2026-06-05T23:41:00.704Z2026-05-23T03:13:17.425ZS-EPMC126833754136756710.21037/tlcr-2025-717