{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Ilievski F"],"funding":["European Research Council"],"pubmed_abstract":["Single-molecule tracking (SMT) is a powerful tool for real-time studies of protein interactions in living cells. Dye-labelled SNAP-tag and HaloTag self-labelling proteins have simplified SMT significantly, due to their superior photophysical properties compared to fluorescent proteins. However, due to their size, fusion of these tags to a protein of interest often results in loss of protein function. We introduce FLORENCE - a universal labelling method for SMT, based on genetic code expansion (GCE). We overcome significant caveats related to re-coded strains, vectors, and dyes and report successful tracking of site-specifically intracellularly labelled proteins in genomically re-coded <i>E. coli.</i> Our findings establish a robust <i>in vivo</i> protein-labelling strategy, expanding the c"],"journal":["RSC chemical biology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12684252"],"repository":["biostudies-literature"],"pubmed_title":["Optimization of the genetic code expansion technology for intracellular labelling and single-molecule tracking of proteins in genomically re-coded &lt;i&gt;E. coli&lt;/i&gt;."],"pmcid":["PMC12684252"],"funding_grant_id":["947747"],"pubmed_authors":["Borg A","Ilievski F","Brandis G","Johansson M","Wikstrom L","Volkov IL"],"additional_accession":[]},"is_claimable":false,"name":"Optimization of the genetic code expansion technology for intracellular labelling and single-molecule tracking of proteins in genomically re-coded &lt;i&gt;E. coli&lt;/i&gt;.","description":"Single-molecule tracking (SMT) is a powerful tool for real-time studies of protein interactions in living cells. Dye-labelled SNAP-tag and HaloTag self-labelling proteins have simplified SMT significantly, due to their superior photophysical properties compared to fluorescent proteins. However, due to their size, fusion of these tags to a protein of interest often results in loss of protein function. We introduce FLORENCE - a universal labelling method for SMT, based on genetic code expansion (GCE). We overcome significant caveats related to re-coded strains, vectors, and dyes and report successful tracking of site-specifically intracellularly labelled proteins in genomically re-coded <i>E. coli.</i> Our findings establish a robust <i>in vivo</i> protein-labelling strategy, expanding the c","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-14T03:23:40.233Z","creation":"2026-06-14T03:09:20.748Z"},"accession":"S-EPMC12684252","cross_references":{"pubmed":["41368476"],"doi":["10.1039/d5cb00221d"]}}