biostudies-literatureUnknown15(1)Ibrahim IAAin Shams UniversityMango peel waste is a rich source of bioactive compounds with potential for sustainable valorization. In this study, coarse and fine mango peel powders were subjected to ethanolic and supercritical CO₂ extraction. The resulting extracts were evaluated for phenolic and flavonoid content, antioxidant activity (DPPH and FRAP), as well as antibacterial, antiadhesion, and antibiofilm properties against six multidrug-resistant foodborne pathogens. The ethanolic extract from fine mango peel powder (EMPF) exhibited the highest phenolic (2488 µg GA eq/mL) and flavonoid (1282 µg rutin eq/mL) content, with quercetin (63.75 mg/L) and p-hydroxybenzoic acid (28.68 mg/L) as major compounds, and showed strong antioxidant activity (RSA = 40%, FRAP = 0.804). To enhance stability and bioactivity, EMPF was naScientific reports43283https://www.ebi.ac.uk/biostudies/studies/S-EPMC12686472biostudies-literatureSustainable valorization of mango peel waste by extracting bioactive compounds for functional applications.PMC12686472Heikal YAEIbrahim IAEbeid HMMElhariry HMfalseSustainable valorization of mango peel waste by extracting bioactive compounds for functional applications.Mango peel waste is a rich source of bioactive compounds with potential for sustainable valorization. In this study, coarse and fine mango peel powders were subjected to ethanolic and supercritical CO₂ extraction. The resulting extracts were evaluated for phenolic and flavonoid content, antioxidant activity (DPPH and FRAP), as well as antibacterial, antiadhesion, and antibiofilm properties against six multidrug-resistant foodborne pathogens. The ethanolic extract from fine mango peel powder (EMPF) exhibited the highest phenolic (2488 µg GA eq/mL) and flavonoid (1282 µg rutin eq/mL) content, with quercetin (63.75 mg/L) and p-hydroxybenzoic acid (28.68 mg/L) as major compounds, and showed strong antioxidant activity (RSA = 40%, FRAP = 0.804). To enhance stability and bioactivity, EMPF was na2025-01-01T00:00:00Z2025 Dec2026-06-07T05:27:51.804Z2026-06-07T03:07:22.442ZS-EPMC126864724135465410.1038/s41598-025-28141-z