{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Onal M"],"funding":["National Institutes of Health","University of Arkansas for Medical Sciences","NIH HHS"],"pagination":["e70256"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12686594"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(12)"],"pubmed_abstract":["A decrease in osteoblast number and bone formation are seminal contributors to age-related osteoporosis. However, the aging-associated molecular mechanisms that impact osteoblast precursors, osteoblasts, osteocytes, and other bone mesenchymal cell types remain unclear. We performed single-cell RNA-sequencing of mesenchymal cells present at the endosteum and periosteum of young and old C57BL/6 mice of both sexes. Osteoblast precursors and osteoblasts from female endosteum exhibited the greatest changes with aging. Transcriptional changes revealed decreased matrix protein production and autophagy, as well as increased senescence, phosphorylation, and hypoxia. Because deficient macroautophagy in osteoblast lineage cells decreases bone formation, we contrasted the transcriptional changes cause"],"journal":["Aging cell"],"pubmed_title":["The Aging Landscape by scRNAseq of Mesenchymal Lineage Cells in Mouse Bone."],"pmcid":["PMC12686594"],"funding_grant_id":["R01AG068449","P20GM125503","R01AR56679"],"pubmed_authors":["Xiong J","Kim HN","Marques-Carvalho A","Almeida M","James A","Kurilung A","O'Brien CA","Nookaew I","Gatrell L","Warren A","Reyes-Castro O","Resende-Coelho A","Onal M"],"additional_accession":[]},"is_claimable":false,"name":"The Aging Landscape by scRNAseq of Mesenchymal Lineage Cells in Mouse Bone.","description":"A decrease in osteoblast number and bone formation are seminal contributors to age-related osteoporosis. However, the aging-associated molecular mechanisms that impact osteoblast precursors, osteoblasts, osteocytes, and other bone mesenchymal cell types remain unclear. We performed single-cell RNA-sequencing of mesenchymal cells present at the endosteum and periosteum of young and old C57BL/6 mice of both sexes. Osteoblast precursors and osteoblasts from female endosteum exhibited the greatest changes with aging. Transcriptional changes revealed decreased matrix protein production and autophagy, as well as increased senescence, phosphorylation, and hypoxia. Because deficient macroautophagy in osteoblast lineage cells decreases bone formation, we contrasted the transcriptional changes cause","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-05T23:56:00.494Z","creation":"2026-05-23T03:13:51.707Z"},"accession":"S-EPMC12686594","cross_references":{"pubmed":["41078084"],"doi":["10.1111/acel.70256"]}}