biostudies-literatureUnknown24(12)Chen XNasopharyngeal carcinoma (NPC) represents a malignant tumor linked to Epstein-Barr virus (EBV) that is characterized by distinctive, clinically relevant biological features. As protein N-glycosylation plays critical roles in cancer progression, we applied quantitative glycoproteomics to characterize NPC-specific glycosylation patterns. Using label-free proteomics and intact N-glycoproteomics, we analyzed 17 NPC tissues and 7 normal mucosal epithelia. Integrated analysis with PET/CT imaging and bioinformatics revealed correlations among differentially expressed glycoproteins, glycosyltransferases, and immunophenotypes. Functional validation demonstrated that MANEA, an enzyme linked to high-mannose glycosylation, influences NPC cell proliferation and migration. Furthermore, MANEA likely moduJournal of proteome research6272-6284https://www.ebi.ac.uk/biostudies/studies/S-EPMC12687360biostudies-literatureProteomics Combined with N-Glycoproteomics to Explore the Pathogenesis of Nasopharyngeal Carcinoma.PMC12687360Lin QLi ZLing HChen XZhou YWu YfalseProteomics Combined with N-Glycoproteomics to Explore the Pathogenesis of Nasopharyngeal Carcinoma.Nasopharyngeal carcinoma (NPC) represents a malignant tumor linked to Epstein-Barr virus (EBV) that is characterized by distinctive, clinically relevant biological features. As protein N-glycosylation plays critical roles in cancer progression, we applied quantitative glycoproteomics to characterize NPC-specific glycosylation patterns. Using label-free proteomics and intact N-glycoproteomics, we analyzed 17 NPC tissues and 7 normal mucosal epithelia. Integrated analysis with PET/CT imaging and bioinformatics revealed correlations among differentially expressed glycoproteins, glycosyltransferases, and immunophenotypes. Functional validation demonstrated that MANEA, an enzyme linked to high-mannose glycosylation, influences NPC cell proliferation and migration. Furthermore, MANEA likely modu2025-01-01T00:00:00Z2025 Dec2026-06-05T23:43:20.676Z2026-05-23T03:13:36.987ZS-EPMC126873604126976710.1021/acs.jproteome.5c00793