{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Cid-Rojas F"],"funding":["NIAID NIH HHS"],"pubmed_abstract":["<i>Clostridioides difficile</i> is a Gram-positive and obligate anaerobic spore-former pathogen. <i>C. difficile</i> spores are essential for the transmission and recurrence of <i>C. difficile</i> infections (CDI). A major challenge in sporulation studies in <i>C. difficile</i> is the low yield and asynchronous nature of this process. In this work, a hypersporulating strain, derivative of R20291, with an early sporulation onset and enhanced sporulation efficiency was isolated by serendipity. This strain had 1000-fold higher sporulation efficiency than the parental R20291 strain in sessile culture conditions. Electron micrographs revealed that spores of both strains have similar hair-like projections, electron-dense outer exosporium layer features. Whole genome sequencing and genomic analys"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.11.25.690273"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12687779"],"repository":["biostudies-literature"],"pubmed_title":["Characterization of a hypersporulating strain derivative &lt;i&gt;of Clostridioides difficile&lt;/i&gt; R20291."],"pmcid":["PMC12687779"],"funding_grant_id":["R01 AI177842"],"pubmed_authors":["Cid-Rojas F","Paredes-Sabja D"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of a hypersporulating strain derivative &lt;i&gt;of Clostridioides difficile&lt;/i&gt; R20291.","description":"<i>Clostridioides difficile</i> is a Gram-positive and obligate anaerobic spore-former pathogen. <i>C. difficile</i> spores are essential for the transmission and recurrence of <i>C. difficile</i> infections (CDI). A major challenge in sporulation studies in <i>C. difficile</i> is the low yield and asynchronous nature of this process. In this work, a hypersporulating strain, derivative of R20291, with an early sporulation onset and enhanced sporulation efficiency was isolated by serendipity. This strain had 1000-fold higher sporulation efficiency than the parental R20291 strain in sessile culture conditions. Electron micrographs revealed that spores of both strains have similar hair-like projections, electron-dense outer exosporium layer features. Whole genome sequencing and genomic analys","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-04T03:15:55.251Z","creation":"2026-06-04T03:11:13.125Z"},"accession":"S-EPMC12687779","cross_references":{"pubmed":["41377515"],"doi":["10.1101/2025.11.25.690273"]}}