{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Okafornta CW"],"funding":["NIH HHS"],"pubmed_abstract":["How embryos adapt their internal cellular machinery to reductions in cell size during development remains a fundamental question in cell biology<sup>1-11</sup>. Here, we use high-resolution lattice light-sheet fluorescence microscopy and automated image analysis to quantify lineage-resolved mitotic spindle and chromosome segregation dynamics from the 2- to 64-cell stages in <i>Caenorhabditis elegans</i> embryos. While spindle length scales with cell size across both wild-type and size-perturbed embryos, chromosome segregation dynamics remain largely invariant, suggesting that distinct mechanisms govern these mitotic processes. Combining femtosecond laser ablation<sup>12,13</sup> with large-scale electron tomography<sup>14</sup>, we find that central spindle microtubules mediate chromosome "],"journal":["Research square"],"pagination":["rs.3.rs-7923379"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12687828"],"repository":["biostudies-literature"],"pubmed_title":["Cell size reduction scales spindle elongation but not chromosome segregation in &lt;i&gt;C. elegans&lt;/i&gt;."],"pmcid":["PMC12687828"],"funding_grant_id":["P40 OD010440"],"pubmed_authors":["Farhadifar R","Okafornta CW","Muller-Reichert T","Baum D","Needleman DJ","Kockert M","Fabig G","Wu HY","Vogel M","Haase R","Shelley MJ"],"additional_accession":[]},"is_claimable":false,"name":"Cell size reduction scales spindle elongation but not chromosome segregation in &lt;i&gt;C. elegans&lt;/i&gt;.","description":"How embryos adapt their internal cellular machinery to reductions in cell size during development remains a fundamental question in cell biology<sup>1-11</sup>. Here, we use high-resolution lattice light-sheet fluorescence microscopy and automated image analysis to quantify lineage-resolved mitotic spindle and chromosome segregation dynamics from the 2- to 64-cell stages in <i>Caenorhabditis elegans</i> embryos. While spindle length scales with cell size across both wild-type and size-perturbed embryos, chromosome segregation dynamics remain largely invariant, suggesting that distinct mechanisms govern these mitotic processes. Combining femtosecond laser ablation<sup>12,13</sup> with large-scale electron tomography<sup>14</sup>, we find that central spindle microtubules mediate chromosome ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-04T03:21:18.695Z","creation":"2026-06-04T03:12:44.147Z"},"accession":"S-EPMC12687828","cross_references":{"pubmed":["41377964"],"doi":["10.21203/rs.3.rs-7923379/v1"]}}