biostudies-literatureUnknown9(12)Ghafoor MSNucleoredoxin 1 (NRX1), a member of the redoxin superfamily, plays a critical role in maintaining redox homeostasis and enhancing stress tolerance in plants. We employed integrated in silico analyses and CRISPR-Cas9-based genome editing to functionally characterize NRX1 in <i>Triticum aestivum</i> (wheat) responding to salinity and infection by <i>Puccinia striiformis</i>. We identified five NRX1 proteins coded by three homeologs, with each containing conserved thioredoxin-like domains and a Cys-rich C-terminal region. Sequence analysis predicted cytosolic and chloroplast localization, and promoter analysis predicted interaction with numerous <i>cis</i>-regulatory elements responsive to stress and hormones, including ABRE, MeJARE, and LTRE motifs. Expression profiling revealed significant Plant directe70130https://www.ebi.ac.uk/biostudies/studies/S-EPMC12688404biostudies-literatureNucleoredoxin 1 in Wheat: Genomic Analysis and Demonstration of Its Role in Redox Homeostasis and Stress Resilience.PMC12688404Naz RHussain ANosheen AShier WTGhafoor MSYasmin HSajjad MKeyani RfalseNucleoredoxin 1 in Wheat: Genomic Analysis and Demonstration of Its Role in Redox Homeostasis and Stress Resilience.Nucleoredoxin 1 (NRX1), a member of the redoxin superfamily, plays a critical role in maintaining redox homeostasis and enhancing stress tolerance in plants. We employed integrated in silico analyses and CRISPR-Cas9-based genome editing to functionally characterize NRX1 in <i>Triticum aestivum</i> (wheat) responding to salinity and infection by <i>Puccinia striiformis</i>. We identified five NRX1 proteins coded by three homeologs, with each containing conserved thioredoxin-like domains and a Cys-rich C-terminal region. Sequence analysis predicted cytosolic and chloroplast localization, and promoter analysis predicted interaction with numerous <i>cis</i>-regulatory elements responsive to stress and hormones, including ABRE, MeJARE, and LTRE motifs. Expression profiling revealed significant 2025-01-01T00:00:00Z2025 Dec2026-06-06T01:07:00.909Z2026-05-24T03:11:46.781ZS-EPMC126884044137783910.1002/pld3.70130