{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang Y"],"funding":["Fundamental Research Funds for the Central Universities, Nankai University","National Science Foundation of China","Tianjin Municipal Science and Technology Committee (Tianjin Municipal Science and Technology Commission)","Tianjin Municipal Science and Technology Committee"],"pagination":["e0278425"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12691604"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["16(12)"],"pubmed_abstract":["<i>Pseudomonas aeruginosa</i> is an environmental bacterium and opportunistic human pathogen capable of causing a wide range of infections in humans. The type III secretion system (T3SS) plays a key role in the pathogenesis of acute <i>P. aeruginosa</i> infections. Expression of the T3SS is controlled by a master activator ExsA, whose transcription is driven by its operon promoter P<i><sub>exsC</sub></i> as well as its own promoter P<i><sub>exsA</sub></i>. Here, we found that plasmid replication initiation protein TrfA acts as a repressor for the T3SS gene expression in <i>P. aeruginosa</i>. Production of the TrfA reduced the expression of the T3SS genes and the bacterial pathogenicity in a mouse acute pneumonia model. Further analysis revealed that the expression of <i>trfA</i> resulted i"],"journal":["mBio"],"pubmed_title":["Plasmid replication initiator protein TrfA represses the host type III secretion system in &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt;."],"pmcid":["PMC12691604"],"funding_grant_id":["22JCYBJC00790","63231048","63231047","22JCYBJC00560","32170177","32170199"],"pubmed_authors":["Zhang Y","Jin S","Bian Y","Liu Q","Shen C","Ma Z","Ha U-H","Wu W","Jin Y","Yin L","Wang L","Cheng Z"],"additional_accession":[]},"is_claimable":false,"name":"Plasmid replication initiator protein TrfA represses the host type III secretion system in &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt;.","description":"<i>Pseudomonas aeruginosa</i> is an environmental bacterium and opportunistic human pathogen capable of causing a wide range of infections in humans. The type III secretion system (T3SS) plays a key role in the pathogenesis of acute <i>P. aeruginosa</i> infections. Expression of the T3SS is controlled by a master activator ExsA, whose transcription is driven by its operon promoter P<i><sub>exsC</sub></i> as well as its own promoter P<i><sub>exsA</sub></i>. Here, we found that plasmid replication initiation protein TrfA acts as a repressor for the T3SS gene expression in <i>P. aeruginosa</i>. Production of the TrfA reduced the expression of the T3SS genes and the bacterial pathogenicity in a mouse acute pneumonia model. Further analysis revealed that the expression of <i>trfA</i> resulted i","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-06T00:12:29.118Z","creation":"2026-05-23T03:13:58.57Z"},"accession":"S-EPMC12691604","cross_references":{"pubmed":["41190656"],"doi":["10.1128/mbio.02784-25"]}}