biostudies-literatureRivera JCNIMHD NIH HHSCalifornia TRDRPNIMHD AXISNIH R25NIGMS NIH HHSDOD CDMRP11491https://www.ebi.ac.uk/biostudies/studies/S-EPMC12691771biostudies-literatureUnknown26(23)Electronic cigarettes (E-Cig) are a new way of delivering nicotine, gaining popularity among adolescents and young adults, who often do not realize their harmful effects. Although the adverse effects of E-Cigs on the liver and heart have been demonstrated, their effects on the skeletal muscle have not been well studied. In this study, we evaluated the skeletal muscle effects of E-Cig aerosol, delivered in a manner similar to human vaping, in a mouse model of obesity induced by a high-fat diet (HFD). C57BL/6 mice, fed either a normal chow diet (NCD) or HFD, were exposed to either saline aerosol control or aerosol generated from Blu PLUS^TM containing 0% or 2.4% nicotine for 12 weeks. Mice fed an NCD were included to distinguish whether E-Cig effects on the skeletal muscle requiredInternational journal of molecular sciencesElectronic Cigarette Exposure Induces Adverse Cellular Alterations in Skeletal Muscle in Male Mice Subjected to a High-Fat Diet.PMC1269177128CP-0040SC2GM125551DA050723PR190942S21-MD000103U54MD007598SC2GM135127Wilson JTintut YJordan MCSinha-Hikim APFriedman TCLiu YShao XMRivera JCEspinoza-Derout JLao CJHasan KPuri VRoos KPfalseElectronic Cigarette Exposure Induces Adverse Cellular Alterations in Skeletal Muscle in Male Mice Subjected to a High-Fat Diet.Electronic cigarettes (E-Cig) are a new way of delivering nicotine, gaining popularity among adolescents and young adults, who often do not realize their harmful effects. Although the adverse effects of E-Cigs on the liver and heart have been demonstrated, their effects on the skeletal muscle have not been well studied. In this study, we evaluated the skeletal muscle effects of E-Cig aerosol, delivered in a manner similar to human vaping, in a mouse model of obesity induced by a high-fat diet (HFD). C57BL/6 mice, fed either a normal chow diet (NCD) or HFD, were exposed to either saline aerosol control or aerosol generated from Blu PLUS^TM containing 0% or 2.4% nicotine for 12 weeks. Mice fed an NCD were included to distinguish whether E-Cig effects on the skeletal muscle required2025-01-01T00:00:00Z2025 Nov2026-05-26T17:06:14.312Z2026-05-24T03:11:15.678ZS-EPMC126917714137364610.3390/ijms262311491