{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["von Bernhardi R"],"funding":["Fondo Nacional de Desarrollo Científico y Tecnológico"],"pagination":["11378"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12692065"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(23)"],"pubmed_abstract":["Ageing results in an increased microglial activation and neuroinflammation. We are interested in assessing ageing-dependent changes in the amount and fractalkine (CX3CL1) proteoforms participating in neuron-microglia crosstalk that could be involved in microglia activation. We analysed age-dependent changes in CX3CL1, CX3CR1, and TGFβ mRNAs using RT-qPCR and CX3CL1 proteoforms using Western blot, in 3 to 20-month-old WT mice and an inflammatory mouse model (SRA<sup>-/-</sup>) treated with 0.5 mg/kg of intraperitoneal LPS, 2 ng of intrathecal TGFβ, or a vehicle. CX3CL1, CX3CR1, and TGFβ were affected by ageing. CX3CL1 mRNA was similar in young and adult mice but decreased by 52% in >20-month-old mice; adult mice showed a 3-fold increase in 70 kDa soluble CX3CL1. CX3CR1 showed a progressive "],"journal":["International journal of molecular sciences"],"pubmed_title":["Age-Related Changes in Neuron-Microglia Interaction Mediated by Fractalkine Under Inflammatory Conditions."],"pmcid":["PMC12692065"],"funding_grant_id":["1221028"],"pubmed_authors":["Godoy G","Eugenin J","von Bernhardi R","Metz C","Cortes F","Narea C"],"additional_accession":[]},"is_claimable":false,"name":"Age-Related Changes in Neuron-Microglia Interaction Mediated by Fractalkine Under Inflammatory Conditions.","description":"Ageing results in an increased microglial activation and neuroinflammation. We are interested in assessing ageing-dependent changes in the amount and fractalkine (CX3CL1) proteoforms participating in neuron-microglia crosstalk that could be involved in microglia activation. We analysed age-dependent changes in CX3CL1, CX3CR1, and TGFβ mRNAs using RT-qPCR and CX3CL1 proteoforms using Western blot, in 3 to 20-month-old WT mice and an inflammatory mouse model (SRA<sup>-/-</sup>) treated with 0.5 mg/kg of intraperitoneal LPS, 2 ng of intrathecal TGFβ, or a vehicle. CX3CL1, CX3CR1, and TGFβ were affected by ageing. CX3CL1 mRNA was similar in young and adult mice but decreased by 52% in >20-month-old mice; adult mice showed a 3-fold increase in 70 kDa soluble CX3CL1. CX3CR1 showed a progressive ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-05-26T11:08:45.79Z","creation":"2026-05-24T03:11:29.993Z"},"accession":"S-EPMC12692065","cross_references":{"pubmed":["41373544"],"doi":["10.3390/ijms262311378"]}}