{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Area-Navarro M"],"funding":["Agència de Gestió d'Ajuts Universitaris i de Recerca","Universitat Autònoma de Barcelona"],"pagination":["11357"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12692271"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(23)"],"pubmed_abstract":["HEK-293 is a highly transfectable human cell line widely used as a model for protein expression. Since large amounts of cells are often required for the purification of HLA immunopeptidomes, suspension-growing variants, such as HEK-293F, facilitate the generation of sufficient cell quantities. The HLA class I-typing of these cells is <i>HLA-A*02:01</i>, -<i>A*03:01</i>, -<i>B*07:02</i>, and -<i>C*07:02</i>. HEK-293T cells have been previously used as a source of HLA peptide ligands derived from SARS-CoV-2 proteins. In this study, we purified and analyzed the HLA-I immunopeptidome of HEK-293 and HEK-293F cells using mass spectrometry. Cell surface expression of specific HLA-I allotypes was determined using flow cytometry with allele-specific antibodies. The HLA-I immunopeptidome of HEK-293 "],"journal":["International journal of molecular sciences"],"pubmed_title":["Specific Instability of HLA-A*03:01 Expression in HEK-293 Cells."],"pmcid":["PMC12692271"],"funding_grant_id":["PPC2024-38","2020PANDE00079"],"pubmed_authors":["Juan M","Alvarez I","Palacio JR","Area-Navarro M","Canals F","Tirado-Herranz A","Cerqueira A","Pastor-Moreno A","Scholz E","Marcilla M"],"additional_accession":[]},"is_claimable":false,"name":"Specific Instability of HLA-A*03:01 Expression in HEK-293 Cells.","description":"HEK-293 is a highly transfectable human cell line widely used as a model for protein expression. Since large amounts of cells are often required for the purification of HLA immunopeptidomes, suspension-growing variants, such as HEK-293F, facilitate the generation of sufficient cell quantities. The HLA class I-typing of these cells is <i>HLA-A*02:01</i>, -<i>A*03:01</i>, -<i>B*07:02</i>, and -<i>C*07:02</i>. HEK-293T cells have been previously used as a source of HLA peptide ligands derived from SARS-CoV-2 proteins. In this study, we purified and analyzed the HLA-I immunopeptidome of HEK-293 and HEK-293F cells using mass spectrometry. Cell surface expression of specific HLA-I allotypes was determined using flow cytometry with allele-specific antibodies. The HLA-I immunopeptidome of HEK-293 ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-05-26T11:06:52.608Z","creation":"2026-05-24T03:11:04.91Z"},"accession":"S-EPMC12692271","cross_references":{"pubmed":["41373516"],"doi":["10.3390/ijms262311357"]}}