biostudies-literatureMartinello MRipartizione delle somme assegnate per l'esecuzione del Sottoprogramma nazionale del Piano apistico nazionale e relativo bando per l'accesso ai finanziamenti, per l'annualità 2023" - MASAF Prot. Interno N. 0109691 del 17/02/2023: "Messa a punto e validazi4050https://www.ebi.ac.uk/biostudies/studies/S-EPMC12692283biostudies-literatureUnknown14(23)Glyphosate-based herbicides are among the most widely used pesticides worldwide, but data on their occurrence in food products, particularly honey, remain limited. The analytical determination of glyphosate, its primary metabolite aminomethylphosphonic acid (AMPA), and glufosinate ammonium is technically challenging due to their high polarity and distinctive physicochemical properties, requiring the development of dedicated single-residue analytic methods. In this study, honey samples were prepared by derivatizing the target analytes with 9-fluorenylmethyl chloroformate (FMOC-Cl), followed by solid-phase extraction (SPE) using hydrophilic-lipophilic balanced (HLB) cartridges to improve matrix clean-up and enhance analytical sensitivity. Quantification was performed by liquid chromatographyFoods (Basel, Switzerland)The Development and Validation of an LC-MS/MS Method for the Determination of Glyphosate, AMPA, and Glufosinate in Honey Following FMOC-Cl Derivatization: Application to Italian Samples.PMC12692283J28H23000340001Martinello MMutinelli FZanella SBertola MfalseThe Development and Validation of an LC-MS/MS Method for the Determination of Glyphosate, AMPA, and Glufosinate in Honey Following FMOC-Cl Derivatization: Application to Italian Samples.Glyphosate-based herbicides are among the most widely used pesticides worldwide, but data on their occurrence in food products, particularly honey, remain limited. The analytical determination of glyphosate, its primary metabolite aminomethylphosphonic acid (AMPA), and glufosinate ammonium is technically challenging due to their high polarity and distinctive physicochemical properties, requiring the development of dedicated single-residue analytic methods. In this study, honey samples were prepared by derivatizing the target analytes with 9-fluorenylmethyl chloroformate (FMOC-Cl), followed by solid-phase extraction (SPE) using hydrophilic-lipophilic balanced (HLB) cartridges to improve matrix clean-up and enhance analytical sensitivity. Quantification was performed by liquid chromatography2025-01-01T00:00:00Z2025 Nov2026-05-26T17:05:09.43Z2026-05-24T03:11:15.638ZS-EPMC126922834137598810.3390/foods14234050