biostudies-literatureUnknown26(23)Park BOExtracellular vesicles (EVs) derived from probiotic bacteria have recently emerged as postbiotic mediators that regulate host cellular responses. This study investigated the effects of EVs from <i>Lactobacillus rhamnosus BS-Pro-08</i>, isolated from kefir grains (Lacto EV), on adipocyte differentiation and lipid metabolism. Lacto EV treatment markedly suppressed the differentiation of 3T3-L1 preadipocytes into mature adipocytes, as reflected by reduced lipid accumulation and decreased expression of the adipogenic transcription factors peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα). This inhibitory effect was most pronounced at the early stage of adipogenesis. In mature adipocytes, Lacto EV enhanced lipolysis in a dose-dependent manner, acInternational journal of molecular sciences11732https://www.ebi.ac.uk/biostudies/studies/S-EPMC12692708biostudies-literatureExtracellular Vesicles from &lt;i&gt;Lactobacillus rhamnosus BS-Pro-08&lt;/i&gt;, Kefir Grain, Suppress Adipogenesis and Enhance Lipolysis in Adipocytes.PMC12692708Shin SPark BOSim JJung EYeom MWang HSong CHLee HWCho EPark DfalseExtracellular Vesicles from &lt;i&gt;Lactobacillus rhamnosus BS-Pro-08&lt;/i&gt;, Kefir Grain, Suppress Adipogenesis and Enhance Lipolysis in Adipocytes.Extracellular vesicles (EVs) derived from probiotic bacteria have recently emerged as postbiotic mediators that regulate host cellular responses. This study investigated the effects of EVs from <i>Lactobacillus rhamnosus BS-Pro-08</i>, isolated from kefir grains (Lacto EV), on adipocyte differentiation and lipid metabolism. Lacto EV treatment markedly suppressed the differentiation of 3T3-L1 preadipocytes into mature adipocytes, as reflected by reduced lipid accumulation and decreased expression of the adipogenic transcription factors peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα). This inhibitory effect was most pronounced at the early stage of adipogenesis. In mature adipocytes, Lacto EV enhanced lipolysis in a dose-dependent manner, ac2025-01-01T00:00:00Z2025 Dec2026-05-26T14:05:54.592Z2026-05-24T03:11:13.555ZS-EPMC126927084137387710.3390/ijms262311732