biostudies-literatureFekete-Kertesz IHungarian National Research, Development and Innovation Office - Scientific Research ProgrammesHORIZON-MSCA-2022-SE-01-01Hungarian Academy of Sciences, János Bolyai Research ScholarshipMinistry of Culture and Innovation of Hungary from the National Research, Development and Innovation Fund1800https://www.ebi.ac.uk/biostudies/studies/S-EPMC12693538biostudies-literatureUnknown15(23)As the exposure of the aquatic ecosystem to graphene oxide (GO) increases with its growing production and use, understanding the structure-property-toxicity relationships becomes increasingly critical in the development of effective safe design guidelines. An appropriate testing methodology is crucial in ecotoxicity assessments to accurately characterize the environmentally relevant toxicity of nanoparticles, particularly for GO, where the physicochemical properties fundamentally determine their interactions and toxicity toward aquatic organisms. Many ecotoxicological methods require the heat sterilization of samples as a preliminary treatment prior to analysis. To investigate changes in toxicity profiles induced by wet heat sterilization pretreatments (autoclaving and Tyndall treatment) oNanomaterials (Basel, Switzerland)Pre-Experimental Wet Heat Sterilization Alters the Ecotoxicity of Pristine Graphene Oxide Toward <i>Daphnia magna</i>.PMC12693538BO/00276/25TKP2021-EGA-02CLEANWATER #101131382OTKA PD_146326OTKA K_143571Podhragyai VLaszlo KMolnar MHajdinak PMolnar ZBulatko AFekete-Kertesz IGyarmati BSfalsePre-Experimental Wet Heat Sterilization Alters the Ecotoxicity of Pristine Graphene Oxide Toward <i>Daphnia magna</i>.As the exposure of the aquatic ecosystem to graphene oxide (GO) increases with its growing production and use, understanding the structure-property-toxicity relationships becomes increasingly critical in the development of effective safe design guidelines. An appropriate testing methodology is crucial in ecotoxicity assessments to accurately characterize the environmentally relevant toxicity of nanoparticles, particularly for GO, where the physicochemical properties fundamentally determine their interactions and toxicity toward aquatic organisms. Many ecotoxicological methods require the heat sterilization of samples as a preliminary treatment prior to analysis. To investigate changes in toxicity profiles induced by wet heat sterilization pretreatments (autoclaving and Tyndall treatment) o2025-01-01T00:00:00Z2025 Nov2026-05-23T03:27:07.567Z2026-05-23T03:14:06.676ZS-EPMC126935384136947110.3390/nano15231800