biostudies-literatureJackson DJNIDCR NIH HHSJohns Hopkins Cell Biology AwardNIHNIH HHSiyaf214https://www.ebi.ac.uk/biostudies/studies/S-EPMC12693586biostudies-literatureUnknown231(4)DNA binding assays, expression analyses, and binding site mutagenesis revealed that the Drosophila CrebA transcription factor (TF) boosts secretory capacity in the embryonic salivary gland through direct regulation of secretory pathway component genes (SPCGs). The mammalian orthologues of CrebA, the Creb3L-family of leucine zipper TFs, not only activate SPCG expression in a variety of mammalian tissues but can also activate SPCG expression in Drosophila embryos, suggesting a highly conserved role for this family of proteins in boosting secretory capacity. However, in vivo assays reveal that CrebA binds far more genes than it regulates, and it remains unclear what distinguishes functional binding. It is also unclear if CrebA is the major factor driving SPCG gene expression in all DrosophilaGeneticsCrebA regulation of secretory capacity: genome-wide transcription profiling coupled with in vivo DNA binding studies.PMC12693586R01 DE013899RO1 DE013899Jackson DJPeng DAndrew DJHolenarasipura AAjay VCahan PShinde SAfalseCrebA regulation of secretory capacity: genome-wide transcription profiling coupled with in vivo DNA binding studies.DNA binding assays, expression analyses, and binding site mutagenesis revealed that the Drosophila CrebA transcription factor (TF) boosts secretory capacity in the embryonic salivary gland through direct regulation of secretory pathway component genes (SPCGs). The mammalian orthologues of CrebA, the Creb3L-family of leucine zipper TFs, not only activate SPCG expression in a variety of mammalian tissues but can also activate SPCG expression in Drosophila embryos, suggesting a highly conserved role for this family of proteins in boosting secretory capacity. However, in vivo assays reveal that CrebA binds far more genes than it regulates, and it remains unclear what distinguishes functional binding. It is also unclear if CrebA is the major factor driving SPCG gene expression in all Drosophila2025-01-01T00:00:00Z2025 Dec2026-06-09T05:04:03.224Z2026-06-09T03:07:24.447ZS-EPMC126935864105278010.1093/genetics/iyaf214