{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Lu CH"],"funding":["NHLBI NIH HHS","NINDS NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Inside-out activation of integrins is crucial for transducing mechanical forces through the extracellular ligand-integrin-talin-F-actin axis. Extensive studies have shown that talin is the essential player in this process by binding to the intracellular tail of β integrins. Here, we show that, while talin binding is essential for inside-out integrin activation in focal adhesions, it is dispensable in curved adhesions - a distinct adhesion architecture that is exclusively mediated by integrin αvβ5 and selectively formed at curved membranes. Instead, a curvature-sensing protein FCHo2 binds to the HDRRE motif in the cytoplasmic tail of integrin β5 (ITGβ5) and inside-out activates integrin αvβ5 in curved adhesions. Intriguingly, FCHo2 does not bind to a similar motif in the homologous integrin"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2025.11.28.691229"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12694587"],"repository":["biostudies-literature"],"pubmed_title":["FCHo2, instead of talin, enables inside-out activation of integrin ɑvβ5 in curved adhesions."],"pmcid":["PMC12694587"],"funding_grant_id":["T32 GM139791","R35 GM141598","R01 NS121934","T32 GM136568","R01 HL165491"],"pubmed_authors":["Zhang W","Yang Y","Tsai CT","Lu CH","Valencia LA","Lee CE","You H","Cui B"],"additional_accession":[]},"is_claimable":false,"name":"FCHo2, instead of talin, enables inside-out activation of integrin ɑvβ5 in curved adhesions.","description":"Inside-out activation of integrins is crucial for transducing mechanical forces through the extracellular ligand-integrin-talin-F-actin axis. Extensive studies have shown that talin is the essential player in this process by binding to the intracellular tail of β integrins. Here, we show that, while talin binding is essential for inside-out integrin activation in focal adhesions, it is dispensable in curved adhesions - a distinct adhesion architecture that is exclusively mediated by integrin αvβ5 and selectively formed at curved membranes. Instead, a curvature-sensing protein FCHo2 binds to the HDRRE motif in the cytoplasmic tail of integrin β5 (ITGβ5) and inside-out activates integrin αvβ5 in curved adhesions. Intriguingly, FCHo2 does not bind to a similar motif in the homologous integrin","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-04T03:18:36.045Z","creation":"2026-06-04T03:12:08.461Z"},"accession":"S-EPMC12694587","cross_references":{"pubmed":["41383757"],"doi":["10.1101/2025.11.28.691229"]}}