{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Duran Meza AL"],"funding":["National Science Foundation"],"pagination":["e0336376"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12694869"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(12)"],"pubmed_abstract":["The capsid protein of cowpea chlorotic mottle virus (CCMV) has been used extensively for in vitro packaging of heterologous RNA. The associated virus-like particles (VLPs) are spherical with a 3nm-thick/28nm-diameter protein shell and are therefore limited in the amount of RNA they can package. As shown in earlier work, when RNA lengths are longer than ~3500nt the RNA is no longer self-assembled exclusively into a single VLP. Rather, it is shared by two or more 28nm-diameter capsids in the form of doublets, triplets, and higher-order multiplets, with the RNA threaded through the naturally- occurring ~1.5nm-diameter holes in the 180-subunit/icosahedrally-symmetric protein shells. Consistent with this fact we find in the present work that 3500nt is the maximum length of packaged RNA that is "],"journal":["PloS one"],"pubmed_title":["How much genetic information in RNA form can be protected by a CCMV virus-like particle?"],"pmcid":["PMC12694869"],"funding_grant_id":["MCB 2103700"],"pubmed_authors":["Porak SC","Gelbart WM","Knobler CM","Chapman A","Duran Meza AL"],"additional_accession":[]},"is_claimable":false,"name":"How much genetic information in RNA form can be protected by a CCMV virus-like particle?","description":"The capsid protein of cowpea chlorotic mottle virus (CCMV) has been used extensively for in vitro packaging of heterologous RNA. The associated virus-like particles (VLPs) are spherical with a 3nm-thick/28nm-diameter protein shell and are therefore limited in the amount of RNA they can package. As shown in earlier work, when RNA lengths are longer than ~3500nt the RNA is no longer self-assembled exclusively into a single VLP. Rather, it is shared by two or more 28nm-diameter capsids in the form of doublets, triplets, and higher-order multiplets, with the RNA threaded through the naturally- occurring ~1.5nm-diameter holes in the 180-subunit/icosahedrally-symmetric protein shells. Consistent with this fact we find in the present work that 3500nt is the maximum length of packaged RNA that is ","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-06T01:24:24.454Z","creation":"2026-05-24T03:11:48.659Z"},"accession":"S-EPMC12694869","cross_references":{"pubmed":["41370351"],"doi":["10.1371/journal.pone.0336376"]}}