biostudies-literatureUnknown16Liu D<h4>Introduction</h4>Colonization of arbuscular mycorrhizal fungi (AMF) can form the root symbiotic network of co-cultured plants roots and hyphae thus promote plant growth. Maize is often intercropped during the harvesting period of tobacco in tobacco-growing areas in China. AMF Colonization has been shown to be an effective approach for regulating the synergistic growth of Nicotiana tabacum and maize.<h4>Methods</h4>In this study, pot experiments were conducted, and samples were analyzed using 16S rDNA and ITS high-throughput sequencing for bacteria and fungi, respectively,and LC-MS/MS widely targeted metabolomics of soil. Differences in microbiota and metabolites in the rhizosphere soil of tobacco and maize, as well as their correlations with the agrochemical properties of soil at the vFrontiers in plant science1683474https://www.ebi.ac.uk/biostudies/studies/S-EPMC12695787biostudies-literatureAMF-mediated rhizospheric interactions by soil microbiota and metabolites in intercropping of tobacco and maize to regulate the soil nutrients.PMC12695787Nian FLiu YLi JZhao PHan NLiu SLiu DYu JYang SDeng XfalseAMF-mediated rhizospheric interactions by soil microbiota and metabolites in intercropping of tobacco and maize to regulate the soil nutrients.<h4>Introduction</h4>Colonization of arbuscular mycorrhizal fungi (AMF) can form the root symbiotic network of co-cultured plants roots and hyphae thus promote plant growth. Maize is often intercropped during the harvesting period of tobacco in tobacco-growing areas in China. AMF Colonization has been shown to be an effective approach for regulating the synergistic growth of Nicotiana tabacum and maize.<h4>Methods</h4>In this study, pot experiments were conducted, and samples were analyzed using 16S rDNA and ITS high-throughput sequencing for bacteria and fungi, respectively,and LC-MS/MS widely targeted metabolomics of soil. Differences in microbiota and metabolites in the rhizosphere soil of tobacco and maize, as well as their correlations with the agrochemical properties of soil at the v2025-01-01T00:00:00Z20252026-06-06T02:26:19.873Z2026-05-24T03:12:53.663ZS-EPMC126957874139389510.3389/fpls.2025.1683474