{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(1)"],"submitter":["Cheng KW"],"pubmed_abstract":["We describe a viral replicon-based CRISPR knockout (KO) screening approach to specifically identify host factors essential for viral replication which are often missed in live virus screens. We benchmark the replicon screening using a stable fluorescent dengue virus type 2 (DENV-2) replicon cell line and successfully identify host genes known to be required for viral DENV-2 replication (e.g., endoplasmic reticulum membrane complex and oligosaccharyltransferase complex components), along with additional genes that have not been reported in prior CRISPR KO screens with DENV-2. We extend this replicon screening approach to chikungunya virus (CHIKV), a positive-sense RNA virus, and Ebola virus (EBOV), a negative-sense RNA virus, and identify distinct sets of genes required for replication of each virus. Our findings indicate that viral replicon-based CRISPR screens are a useful approach to identify host factors essential for replication of diverse viruses and to elucidate potential novel targets for host-directed medical countermeasures."],"journal":["Nature communications"],"pagination":["11028"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12696002"],"repository":["biostudies-literature"],"pubmed_title":["Replicon-based genome-wide CRISPR knockout screening for the identification of host factors involved in viral replication."],"pmcid":["PMC12696002"],"pubmed_authors":["Travisano KA","Markhard AL","Anaya A","Puschnik AS","Kuhn JH","Medicielo JV","Bhatt KD","Anantpadma M","Kistler AL","Peng D","Bhave M","Lembirik S","Njoya L","Cheng KW"],"additional_accession":[]},"is_claimable":false,"name":"Replicon-based genome-wide CRISPR knockout screening for the identification of host factors involved in viral replication.","description":"We describe a viral replicon-based CRISPR knockout (KO) screening approach to specifically identify host factors essential for viral replication which are often missed in live virus screens. We benchmark the replicon screening using a stable fluorescent dengue virus type 2 (DENV-2) replicon cell line and successfully identify host genes known to be required for viral DENV-2 replication (e.g., endoplasmic reticulum membrane complex and oligosaccharyltransferase complex components), along with additional genes that have not been reported in prior CRISPR KO screens with DENV-2. We extend this replicon screening approach to chikungunya virus (CHIKV), a positive-sense RNA virus, and Ebola virus (EBOV), a negative-sense RNA virus, and identify distinct sets of genes required for replication of each virus. Our findings indicate that viral replicon-based CRISPR screens are a useful approach to identify host factors essential for replication of diverse viruses and to elucidate potential novel targets for host-directed medical countermeasures.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-06T00:27:27.772Z","creation":"2026-05-24T03:11:17.219Z"},"accession":"S-EPMC12696002","cross_references":{"pubmed":["41372121"],"doi":["10.1038/s41467-025-65979-3"]}}