biostudies-literatureVespalcova HCentro de Investigación Biomédica en Red de Epidemiología y Salud PúblicaGeneralitat de CatalunyaAgència de Gestió d'Ajuts Universitaris i de RecercaEuropean Research CouncilInstituto de Salud Carlos IIIHorizon 2020 Framework ProgrammeEuropean CommissionEuropean Regional Development Fund109946https://www.ebi.ac.uk/biostudies/studies/S-EPMC12696147biostudies-literatureUnknown206Phthalates and DINCH are non-persistent chemicals widely used in consumer products. In utero exposure to these compounds has been linked to adverse reproductive and long-term health outcomes, potentially through epigenetic changes in the placenta. This study investigated associations between maternal phthalate and DINCH metabolite levels and placental DNA methylation in 469 mother-child pairs from the Barcelona Life Study Cohort (BiSC). Fifteen phthalate and two DINCH metabolites were measured in pooled maternal urine samples collected at 19 and 35 weeks of gestation using liquid chromatography-tandem mass spectrometry (LC-MS/MS. Placental DNA methylation was assessed using the Illumina EPIC array. We applied robust linear regression models to test associations between single exposures at Environment internationalAssociation of in utero exposure to phthalate and DINCH metabolites with placental DNA methylation.PMC126961472017 SGR 826MCIN/AEI/10.13039/501100011033AC18/00006874583CEX2023-0001290-S785994CB06/02/0041Gomez-Roig MDGomez-Herrera LThomsen CSunyer JDadvand PSanchez Garcia OVrijheid MSakhi AKKnox BAguilar-Lacasana SLlurba EVespalcova HCosin-Tomas MBustamante MfalseAssociation of in utero exposure to phthalate and DINCH metabolites with placental DNA methylation.Phthalates and DINCH are non-persistent chemicals widely used in consumer products. In utero exposure to these compounds has been linked to adverse reproductive and long-term health outcomes, potentially through epigenetic changes in the placenta. This study investigated associations between maternal phthalate and DINCH metabolite levels and placental DNA methylation in 469 mother-child pairs from the Barcelona Life Study Cohort (BiSC). Fifteen phthalate and two DINCH metabolites were measured in pooled maternal urine samples collected at 19 and 35 weeks of gestation using liquid chromatography-tandem mass spectrometry (LC-MS/MS. Placental DNA methylation was assessed using the Illumina EPIC array. We applied robust linear regression models to test associations between single exposures at 2025-01-01T00:00:00Z2025 Dec2026-06-06T01:25:06.712Z2026-05-24T03:11:11.875ZS-EPMC126961474127576310.1016/j.envint.2025.109946