biostudies-literatureUnknown41(6)Li QSanofiRecombinant adeno-associated viruses (AAVs) with precise genome editing and cell-virus interaction have become a promising delivery tool for gene therapy. A robust AAV purification process is crucial for ensuring therapeutic efficacy. The challenges of AAV purification process development encompass limited material availability during early-stage development, high cost-of-goods compared to traditional biologics, and short development timelines for the critical first-in-human stages. The key to overcoming these challenges is to leverage high throughput (HTP) methods. In this article, an integrated end-to-end HTP workflow is proposed, utilizing a resin tip as the purification module and incorporating an HTP analytical toolkit on one platform. Purification parameters, including binding capaciBiotechnology progresse70053https://www.ebi.ac.uk/biostudies/studies/S-EPMC12696448biostudies-literatureAccelerating AAV purification process development using high-throughput resin tip module.PMC12696448Benner SLi QMa JHadidi MfalseAccelerating AAV purification process development using high-throughput resin tip module.Recombinant adeno-associated viruses (AAVs) with precise genome editing and cell-virus interaction have become a promising delivery tool for gene therapy. A robust AAV purification process is crucial for ensuring therapeutic efficacy. The challenges of AAV purification process development encompass limited material availability during early-stage development, high cost-of-goods compared to traditional biologics, and short development timelines for the critical first-in-human stages. The key to overcoming these challenges is to leverage high throughput (HTP) methods. In this article, an integrated end-to-end HTP workflow is proposed, utilizing a resin tip as the purification module and incorporating an HTP analytical toolkit on one platform. Purification parameters, including binding capaci2025-01-01T00:00:00Z2025 Nov-Dec2026-06-06T00:35:24.149Z2026-05-24T03:12:03.06ZS-EPMC126964484067884110.1002/btpr.70053