{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Morbe UM"],"funding":["Novo Nordisk Foundation","Leona M. and Harry B. Helmsley Charitable Trust","European Crohn's and Colitis Organisation","Lundbeck Foundation","Louis-Hansen-Foundation"],"pagination":["e20250471"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12697342"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["223(3)"],"pubmed_abstract":["Gut-associated lymphoid tissues (GALT) represent major sites of adaptive immune priming in the intestine, yet our understanding of human GALT diversity and function remains limited. Here, we used single-cell RNA sequencing, flow cytometry, and confocal laser microscopy to map the fibroblast (FB) landscape of human GALT, including that of Peyer's patches (PP), mucosal isolated lymphoid follicles (M-ILF), and submucosal ILF (SM-ILF). We identify CD24 as a marker that distinguishes GALT from other intestinal FB and demonstrate that CD24+ FB consist of distinct subsets that locate within discrete niches. We show that the composition and transcriptional profile of M-ILF and SM-ILF FB differs with SM-ILF FB appearing more focused at providing T cell support. Finally, we find the transcription profile of PP T zone reticular cells to be altered in Crohn's disease and that cells with a GALT FB-like profile can be detected in other chronic inflammatory diseases. Collectively, our findings provide an important framework for understanding GALT diversity and function."],"journal":["The Journal of experimental medicine"],"pubmed_title":["Fibroblast diversity within human gut-associated lymphoid tissues."],"pmcid":["PMC12697342"],"funding_grant_id":["R155-2014-4184","NNF22OC0071681"],"pubmed_authors":["Jakobsen HL","Nos G","Ensmenger MJ","Madsen GR","Junghus FV","Wewer MD","Morbe UM","Brunak S","Agace WW","Vaananen VA","Nielsen OH","Jorgensen PB","Olsen LR","Riis LB"],"additional_accession":[]},"is_claimable":false,"name":"Fibroblast diversity within human gut-associated lymphoid tissues.","description":"Gut-associated lymphoid tissues (GALT) represent major sites of adaptive immune priming in the intestine, yet our understanding of human GALT diversity and function remains limited. Here, we used single-cell RNA sequencing, flow cytometry, and confocal laser microscopy to map the fibroblast (FB) landscape of human GALT, including that of Peyer's patches (PP), mucosal isolated lymphoid follicles (M-ILF), and submucosal ILF (SM-ILF). We identify CD24 as a marker that distinguishes GALT from other intestinal FB and demonstrate that CD24+ FB consist of distinct subsets that locate within discrete niches. We show that the composition and transcriptional profile of M-ILF and SM-ILF FB differs with SM-ILF FB appearing more focused at providing T cell support. Finally, we find the transcription profile of PP T zone reticular cells to be altered in Crohn's disease and that cells with a GALT FB-like profile can be detected in other chronic inflammatory diseases. Collectively, our findings provide an important framework for understanding GALT diversity and function.","dates":{"release":"2026-01-01T00:00:00Z","publication":"2026 Mar","modification":"2026-05-26T14:04:57.996Z","creation":"2026-05-24T03:12:25.448Z"},"accession":"S-EPMC12697342","cross_references":{"pubmed":["41379085"],"doi":["10.1084/jem.20250471"]}}