{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(46)"],"submitter":["Kim SY"],"funding":["Givaudan International SA (Geneva, Switzerland)"],"pubmed_abstract":["The tongue is essential for swallowing, taste perception, and mechanosensation. The anterior and posterior parts of the tongue have region-specific developmental origins and are maintained by adult epithelial stem/progenitor cells. In vitro models that can be used to investigate anterior tongue biology have been lacking. Here, a protocol is developed to generate a long-term expanding organoid model from the adult mouse dorsal anterior tongue. Anterior tongue organoids consist of Lgr6+ cells, Sox2+ stem/progenitor cells, and Hoxc13+ filiform papillae progenitor cells. Furthermore, anterior tongue organoids share region-specific transcriptomic profiles, gene regulatory networks, and signaling pathways with anterior tongue tissue. Anterior tongue organoids can be differentiated into various epithelial cell types, including Merkel-like cells, keratinocytes, and taste bud cells. Gene regulatory network analysis reveals transcriptional programs associated with Krt8+ cell and Krt23+/Sbsn+ keratinocyte differentiation in the organoids. Together, this study provides an in vitro model of mouse dorsal tongue epithelium."],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pagination":["e06738"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12697779"],"repository":["biostudies-literature"],"pubmed_title":["Organoid Modeling of Mouse Anterior Tongue Epithelium Reveals Regional and Cellular Identities."],"pmcid":["PMC12697779"],"pubmed_authors":["Kim SY","Winkel C","Verweij LHG","Lijnzaad P","Breimer GE","Clevers H","Slack J","Margaritis T","van Es JH","Sanders K","Candelli T","Lin L","van de Wetering M"],"additional_accession":[]},"is_claimable":false,"name":"Organoid Modeling of Mouse Anterior Tongue Epithelium Reveals Regional and Cellular Identities.","description":"The tongue is essential for swallowing, taste perception, and mechanosensation. The anterior and posterior parts of the tongue have region-specific developmental origins and are maintained by adult epithelial stem/progenitor cells. In vitro models that can be used to investigate anterior tongue biology have been lacking. Here, a protocol is developed to generate a long-term expanding organoid model from the adult mouse dorsal anterior tongue. Anterior tongue organoids consist of Lgr6+ cells, Sox2+ stem/progenitor cells, and Hoxc13+ filiform papillae progenitor cells. Furthermore, anterior tongue organoids share region-specific transcriptomic profiles, gene regulatory networks, and signaling pathways with anterior tongue tissue. Anterior tongue organoids can be differentiated into various epithelial cell types, including Merkel-like cells, keratinocytes, and taste bud cells. Gene regulatory network analysis reveals transcriptional programs associated with Krt8+ cell and Krt23+/Sbsn+ keratinocyte differentiation in the organoids. Together, this study provides an in vitro model of mouse dorsal tongue epithelium.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-06T01:27:21.067Z","creation":"2026-05-24T03:11:32.268Z"},"accession":"S-EPMC12697779","cross_references":{"pubmed":["41020687"],"doi":["10.1002/advs.202506738"]}}