biostudies-literatureUnknown16Li Y<h4>Aims</h4>To explore the plasma exosomal miRNA expression profiles in patients with abdominal obesity and type 2 diabetes mellitus (T2DM), identify differentially expressed exosomal m4RNAs, and evaluate the potential of selected exosomal miRNAs as biomarkers for abdominal obesity-related T2DM.<h4>Methods</h4>In the screening phase, high-throughput sequencing was used to detect the plasma exosomal miRNA expression profiles of non-abdominal obese individuals with normal glucose metabolism (NAO/NG, N = 4), abdominal obese individuals with normal glucose metabolism (AO/NG, N = 4), and abdominal obese T2DM patients (AO/T2DM, N = 4). Five differentially expressed miRNAs were identified. In the independent validation phase, RT-qPCR was performed to validate the expression of these miRNAs in thFrontiers in endocrinology1656132https://www.ebi.ac.uk/biostudies/studies/S-EPMC12698432biostudies-literaturePlasma-derived exosomal miRNAs as potentially novel biomarkers for type 2 diabetes mellitus with abdominal obesity.PMC12698432Li YFeng LLi ZXing LZheng LWang YFeng RJia JfalsePlasma-derived exosomal miRNAs as potentially novel biomarkers for type 2 diabetes mellitus with abdominal obesity.<h4>Aims</h4>To explore the plasma exosomal miRNA expression profiles in patients with abdominal obesity and type 2 diabetes mellitus (T2DM), identify differentially expressed exosomal m4RNAs, and evaluate the potential of selected exosomal miRNAs as biomarkers for abdominal obesity-related T2DM.<h4>Methods</h4>In the screening phase, high-throughput sequencing was used to detect the plasma exosomal miRNA expression profiles of non-abdominal obese individuals with normal glucose metabolism (NAO/NG, N = 4), abdominal obese individuals with normal glucose metabolism (AO/NG, N = 4), and abdominal obese T2DM patients (AO/T2DM, N = 4). Five differentially expressed miRNAs were identified. In the independent validation phase, RT-qPCR was performed to validate the expression of these miRNAs in th2025-01-01T00:00:00Z20252026-06-06T02:52:17.557Z2026-05-24T03:13:07.574ZS-EPMC126984324139328510.3389/fendo.2025.1656132