{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Latimer JO"],"funding":["Cotton Research and Development Corporation"],"pagination":["e0334700"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12700366"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(12)"],"pubmed_abstract":["Low molecular weight (LMW) soil organic nitrogen (N) can be a significant source of N in commercial cotton (Gossypium hirsutum L.) systems, potentially comprising a meaningful portion of N uptake in Australian irrigated cotton. Cotton obtains the majority of its N from the soil N pool rather than directly from fertiliser-N. Organic N is the major component of the soil N pool. The purpose of this study was to test the ability of G. hirstutum to take up different organic and inorganic N forms using isotopically labelled compounds. This was done in a sand matrix to reduce potential for microbial competition and enable a clearer view of the physiological capacity of the plant to access different N forms. The experiment showed that cotton took up inorganic N (NO3- and NH4+) and organic N (alanine and urea) concurrently, with a slight preference towards inorganic N overall. The uptake mechanism for organic carbon (C) associated with the organic N was also examined, showing that alanine-C was taken up linearly, with a consistent internal 13C:15N ratio suggesting that some alanine was absorbed intact without extracellular deamination. Overall, the experiment demonstrated that G. hirsutum can rapidly and concurrently access different soil N pools, with a slight preference for inorganic N. The uptake mechanisms for organic N and C are complex, differing between compound types, and warrant further investigation. This study expands the list of plants known to utilise organic N to include commercial cotton, with implications for the management of N fertiliser in cotton growing systems."],"journal":["PloS one"],"pubmed_title":["Nitrogen uptake preference of cotton (Gossypium hirsutum L.)."],"pmcid":["PMC12700366"],"funding_grant_id":["CSP1904"],"pubmed_authors":["Latimer JO","Macdonald BCT","Farrell M"],"additional_accession":[]},"is_claimable":false,"name":"Nitrogen uptake preference of cotton (Gossypium hirsutum L.).","description":"Low molecular weight (LMW) soil organic nitrogen (N) can be a significant source of N in commercial cotton (Gossypium hirsutum L.) systems, potentially comprising a meaningful portion of N uptake in Australian irrigated cotton. Cotton obtains the majority of its N from the soil N pool rather than directly from fertiliser-N. Organic N is the major component of the soil N pool. The purpose of this study was to test the ability of G. hirstutum to take up different organic and inorganic N forms using isotopically labelled compounds. This was done in a sand matrix to reduce potential for microbial competition and enable a clearer view of the physiological capacity of the plant to access different N forms. The experiment showed that cotton took up inorganic N (NO3- and NH4+) and organic N (alanine and urea) concurrently, with a slight preference towards inorganic N overall. The uptake mechanism for organic carbon (C) associated with the organic N was also examined, showing that alanine-C was taken up linearly, with a consistent internal 13C:15N ratio suggesting that some alanine was absorbed intact without extracellular deamination. Overall, the experiment demonstrated that G. hirsutum can rapidly and concurrently access different soil N pools, with a slight preference for inorganic N. The uptake mechanisms for organic N and C are complex, differing between compound types, and warrant further investigation. This study expands the list of plants known to utilise organic N to include commercial cotton, with implications for the management of N fertiliser in cotton growing systems.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025","modification":"2026-06-06T02:01:19.64Z","creation":"2026-05-24T03:12:09.575Z"},"accession":"S-EPMC12700366","cross_references":{"pubmed":["41385508"],"doi":["10.1371/journal.pone.0334700"]}}