{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wen K"],"funding":["Guangdong Basic and Applied Basic Research Foundation","Department of Health of the Hong Kong Special Administrative Region Government"],"pagination":["e0013794"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12700421"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(12)"],"pubmed_abstract":["Chikungunya fever (CF) is a mosquito-borne viral disease caused by Chikungunya virus (CHIKV) that is being increasingly reported in previously non-endemic areas, including Guangdong, China. Despite the low mortality rate associated with CF, some patients may develop chronic joint pain that persists for months or years. Moreover, due to the similarities in symptomatology between CHIKV and other arboviruses, clinical diagnosis without laboratory confirmation is inaccurate. The CHIKV envelope protein E1, critical for viral entry and assembly, is a promising diagnostic and therapeutic target. In this study, we expressed the CHIKV E1 protein in Escherichia coli and developed 16 monoclonal antibodies (mAbs) against E1 to establish an enzyme immunoassay (EIA). The EIA specifically detects CHIKV E"],"journal":["PLoS neglected tropical diseases"],"pubmed_title":["Development of monoclonal antibodies against E1 protein of Chikungunya virus."],"pmcid":["PMC12700421"],"funding_grant_id":["2022A1515011132","2023A1515220071, 2020A1515011171"],"pubmed_authors":["Yuan S","Xu M","Xu P","Zhao C","Di B","Cai J","Chan JF","Yang C","Song J","Zhou Z","Wen K","Ding X","To KK","Feng W","Chen Y","Zhan L","Zhou H"],"additional_accession":[]},"is_claimable":false,"name":"Development of monoclonal antibodies against E1 protein of Chikungunya virus.","description":"Chikungunya fever (CF) is a mosquito-borne viral disease caused by Chikungunya virus (CHIKV) that is being increasingly reported in previously non-endemic areas, including Guangdong, China. Despite the low mortality rate associated with CF, some patients may develop chronic joint pain that persists for months or years. Moreover, due to the similarities in symptomatology between CHIKV and other arboviruses, clinical diagnosis without laboratory confirmation is inaccurate. The CHIKV envelope protein E1, critical for viral entry and assembly, is a promising diagnostic and therapeutic target. In this study, we expressed the CHIKV E1 protein in Escherichia coli and developed 16 monoclonal antibodies (mAbs) against E1 to establish an enzyme immunoassay (EIA). The EIA specifically detects CHIKV E","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Dec","modification":"2026-06-06T02:14:36.691Z","creation":"2026-05-24T03:12:36.731Z"},"accession":"S-EPMC12700421","cross_references":{"pubmed":["41343615"],"doi":["10.1371/journal.pntd.0013794"]}}